Magnetic beads are a convenient affinity support for a variety of assays, which allow easy purification of the target without columns or centrifugation. After a binding step in an affinity purification procedure, the magnetic particles are easily and rapidly collected by placing the microcentrifuge tube or reaction vessel next an appropriate rare-earth magnet. MagnaBind™ beads respond rapidly to MagnaBind™ magnets but can be easily dispersed and regathered multiple times (ie. they will not irreversibly aggregate) because they do not have any magnetic memory.
- Available pre-coated with popular affinity ligands or derivatised for covalent attachment of proteins and other specific ligands
- Beads do not irreversibly aggregate because they have no magnetic memory; collect and disperse the beads multiple times, if needed
- Most separations require a short 5 to 10 minute bench-top procedure
MagnaBind™ beads are available pre-coated with Protein A, Protein G, streptavidin, anti-mouse or anti-rabbit antibodies. Activated beads, with either amine or carboxyl groups, are also available for attaching other proteins or affinity ligands to a magnetic particle.
These beads are applicable in cell sorting using either positive or negative selection and protein purification or immunoassays using either direct or indirect methods.
Features of MagnaBind beads:
Composition: Silanized iron oxide
Magnetisation: 25 to 35 EMU/g
Type of magnetisation: Superparamagnetic (no magnetic memory)
Surface area: >100 m²/g
Bead size: 1 to 4 µm diameter
Settling rate: 4% in 30 minutes
Effective density: 2,5 g/ml
Number of beads: 1×10⁸ beads/mg
pH stability: Aqueous solution, above pH 4,0