The DyLight™ Antibody Labeling Kits were specifically developed for fast, efficient labeling of antibodies. Two convenient kit formats are available to accommodate varied labeling requirements. The DyLight™ Antibody Labeling Kits contain all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins.
- Fast – fluorescently label and purify protein in approximately one hour
- Amine-reactive dyes – label virtually any protein
- Pre-measured fluorescent dye – eliminate the time, waste and hassle associated with weighing dye
- Efficient non-reacted dye removal
- Minimal sample dilution
- Spin column format eliminates the need for column preparation, fraction screening and waiting for protein to emerge from column
- Easy protocol
The DyLight™ Microscale Antibody Labeling Kits contain all the necessary components to perform five separate labeling reactions using 100 μg of IgG. The labeling kits use high-performance spin desalting columns to provide exceptional dye removal and antibody recovery.
Invitrogen DyLight™ Antibody Labeling kits enable the creation of fluorescent antibodies that are covalently linked to different DyLight™ fluorophores. The fluorescently labeled antibodies can be used in a wide variety of laboratory applications, including immunofluorescence microscopy, IHC, western blotting, ELISA, flow cytometry, or in vitro and in vivo fluorescent detection strategies.
DyLight™ dyes exhibit higher fluorescence intensity and photostability than CyDye and LI-COR™ dyes in many applications and remain highly fluorescent over a broad pH range (pH 4–9).
Chemical Reactivity: Amine
Labeling Target: Antibodies (General), Proteins (General)