The qScript™ XLT One-Step RT-PCR Kit is a convenient and highly sensitive system for amplification of RNA templates up to 2 kb. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. This system has been optimised to deliver maximum endpoint RT-PCR efficiency, sensitivity, and specificity.
- Tough tested to overcome inhibitors
- Option GelTrack™ Loading Dye included
- Superior reproducibility
- Convenient single tube reaction assembly at room temperature
Each kit comprises:
1×200 µl qScript™ XLT One-Step Reverse Transcriptase (25X concentrated mixture of qScript™ XLT reverse transcriptase and recombinant ribonuclease inhibitor protein)
2×1,25 ml One-Step ToughMix® (2X concentrated reaction buffer containing dNTP's, magnesium chloride, hot start DNA polymerase, and stabilisers)
1×0,4 ml GelTrack™ Loading Dye (50X concentrated mixture of RT-PCR compatible, blue and yellow electrophoresis tracking dyes)
2×1,5 ml nuclease-free water