The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
- Autoinduction media simplifies protocol by eliminating the monitoring and induction steps
- Sterile stock solutions minimise sample handling, significantly reduce the chance of contamination and are convenient – no need to spend extra time weighing out different ingredients
- Simple, flexible and compatible with any conventional glucose-free bacterial growth medium
With Overnight Express™ Autoinduction Systems, a period of cell growth is followed by spontaneous induction of protein expression – without monitoring cell density and without conventional induction with IPTG. The method is based on media components that are metabolised differentially to promote growth to high density and automatically induce protein expression from lac promoters.
The Overnight Express™ Autoinduction System is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones.
Overnight Express™ Autoinduction System 1: Provides high level protein production in pET and other IPTG-inducible bacterial expression systems.
The Overnight Express™ Autoinduction System 1 kit contains three concentrated sterile solutions:
OnEx™ Solution 1: A blend of carbon sources optimised for tightly regulated uninduced growth to high cell density, followed by high-level induction
OnEx™ Solution 2: A concentrated buffer and nitrogen-source bend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
OnEx™ Solution 3: Provides magnesium for maximal cell density