NBT is used in conjunction with BCIP (BIMB1004) for colourimetric detection of alkaline phophatase activity in blotting, immuno-histochemical and cytochemistry techniques.

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Nitrotetrazolium-blå ≥90% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.

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Nitrotetrazolium-blå ≥98.0% (by HPLC, titration analysis) for biochemical research

Supplier: TCI

Nitrotetrazolium-blå ≥98.0% (by HPLC, titration analysis) for biochemical research

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Nitrotetrazolium-blå

Supplier: Biotium

Nitrotetrazolium-blå

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Nitrotetrazolium-blå ≥99%

Supplier: Thermo Fisher Scientific

Nitrotetrazolium-blå ≥99%

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Nitrotetrazolium-blå, Millipore®

Supplier: Merck Millipore (Oncogene)

Nitrotetrazolium-blå, Millipore®

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Nitrotetrazolium-blå, yellow powder

Supplier: MP Biomedicals

p-Nitroblue Tetrazolium Chloride is a substrate for dehydrogenases and other oxidases and precipitating agent for fibrinogen.

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Nitrotetrazolium-blå for molekylærbiologi

Supplier: MP Biomedicals

p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.

p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.