Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Colorimetric Glucose Oxidase Assay Kit
Supplier: AAT BIOQUEST
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.
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Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen.
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Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways.
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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
Supplier: AAT BIOQUEST
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
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Amplite® Colorimetric Xanthine Oxidase Assay Kit
Supplier: AAT BIOQUEST
Xanthine oxidase (XO)is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyse the oxidation of xanthine to uric acid.
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Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.
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Amplite® Fluorimetric Endotoxin Detection Kit
Supplier: AAT BIOQUEST
Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membrane of Gram-negative bacteria.
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Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.
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Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric GAPDH activity assay kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalysed by GAPDH.
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Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Screen Quest™ fluorimetric neutrophil elastase inhibitor screening kit offers a fast, sensitive, and high-throughput method for screening and characterising potential inhibitors of NE.
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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit
Supplier: AAT BIOQUEST
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.
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Amplite® Fluorimetric Lead (II) Ion Quantitation Kit
Supplier: AAT BIOQUEST
Lead is a highly poisonous metal to human beings, especially young children.
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Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*
Supplier: AAT BIOQUEST
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Amplite® Fluorimetric cADP-Ribose Assay Kit
Supplier: AAT BIOQUEST
This cADP-ribose assay can be performed in a convenient 96-well or 384-well microtiter plate.
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CPRG assay kit
Supplier: OZ BIOSCIENCES
CPRG assay kit is designed to quantify low expression level β-Galactosidase.
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Pierce™ Alkaline Phosphatase, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated alkaline phosphatase is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.
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Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit
Supplier: AAT BIOQUEST
Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.
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Amplite® Colorimetric Gama-Glutamyltransferase (GGT) Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Gamma-Glutamyltransferase (GGT) Activity Assay Kit provides an efficient and straightforward method for measuring GGT activity across various sample types.
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Amplite® Fluorimetric DPP4 Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® fluorimetric DPP4 activity assay kit provides a simple, quick, and direct protocol for measuring DPP4 Activity.
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Amplite® Fluorimetric DPP4 Inhibitor Screening Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors.
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Gaussia luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.
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Pierce™ Cypridina Luciferase Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
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Pierce™ Firefly Luc One-Step Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.
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Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT BIOQUEST
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Fluorimetric Ascorbic Acid Assay Kit
Supplier: AAT BIOQUEST
L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense.
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Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT BIOQUEST
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Amplite® Colorimetric Aldehyde Quantitation Kit
Supplier: AAT BIOQUEST
Rapid and accurate measurement of aldehydes is important task for biological research, food industry, chemical research and environmental pollution surveillance.
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Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit
Supplier: AAT BIOQUEST
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.
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Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit
Supplier: AAT BIOQUEST
Superoxide dismutases (SOD) are a class of enzymes that catalyse the dismutation of superoxide into oxygen and hydrogen peroxide.