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274 results for Enzyme Assays

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Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Supplier: AAT BIOQUEST

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.

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Amplite® Fluorimetric Aldehyde Quantitation Kit

Amplite® Fluorimetric Aldehyde Quantitation Kit

Supplier: AAT BIOQUEST

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

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Amplite® Colorimetric Beta-Galactosidase Assay Kit

Amplite® Colorimetric Beta-Galactosidase Assay Kit

Supplier: AAT BIOQUEST

E. coli beta-galactosidase is a 464 kD tetramer.

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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.

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Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*

Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*

Supplier: AAT BIOQUEST

Caspases play important roles in apoptosis and cell signaling.

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Amplite® Fluorimetric Calcium Quantitation Kit  *Red Fluorescence*

Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.

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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Glutamic acid is one of the 20 proteinogenic amino acids.

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Amplite® Luminometric Peroxidase (HRP) Assay Kit

Amplite® Luminometric Peroxidase (HRP) Assay Kit

Supplier: AAT BIOQUEST

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Colorimetric L-Lactate Assay Kit

Amplite® Colorimetric L-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Colorimetric Antioxidant Activity Assay Kit

Amplite® Colorimetric Antioxidant Activity Assay Kit

Supplier: AAT BIOQUEST

Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.

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Serine hydrolase probes, ActivX™ FP

Serine hydrolase probes, ActivX™ FP

Supplier: Thermo Fisher Scientific

The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

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Amplite® Colorimetric Lipase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.

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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Total Sulfide Quantification Kit

Amplite® Total Sulfide Quantification Kit

Supplier: AAT BIOQUEST

Hydrogen sulfide is not usually a health risk at concentrations present in household water.

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Amplite® Fluorimetric Glucose Quantitation Kit

Amplite® Fluorimetric Glucose Quantitation Kit

Supplier: AAT BIOQUEST

Glucose, a monosaccharide, is the most important carbohydrate in biology.

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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.

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Amplite® Colorimetric Tyrosinase Assay Kit

Amplite® Colorimetric Tyrosinase Assay Kit

Supplier: AAT BIOQUEST

Tyrosinase is of great interest to drug discovery, life science research, food industry and cosmetics industry since it plays an important role in the biosynthetic pathway of melanin.

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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.

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Amplite® IR

Amplite® IR

Supplier: AAT BIOQUEST

Our Amplite® IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2.

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Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit

Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit

Supplier: AAT BIOQUEST

Malondialdehyde (MDA) is one of the natural byproducts during lipid peroxidation.

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Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.

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Amplite® Colorimetric Endotoxin Detection Kit

Amplite® Colorimetric Endotoxin Detection Kit

Supplier: AAT BIOQUEST

Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membranes of Gram-negative bacteria.

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Amplite® Fluorimetric Glucose and Sucrose Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® fluorimetric glucose and sucrose assay kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.

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ReadiUse™ NADPH Regenerating Kit

ReadiUse™ NADPH Regenerating Kit

Supplier: AAT BIOQUEST

NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).

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Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

The monitoring of reduced and oxidised glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury.

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Amplite® Colorimetric Calcium Quantitation Kit  *Blue Color*

Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*

Supplier: AAT BIOQUEST

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.

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Beadlite™ Rapid Colorimetric Thiol Quantitation Kit for Nanoparticles

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Beadlite™ Rapid Colorimetric Thiol Quantitation kit provides a robust method to quantify thiol-functionalised nanoparticle with our proprietary Thiolite™ 490 that has its maximum absorbance at ~490 nm.

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CPRG assay kit

CPRG assay kit

Supplier: OZ BIOSCIENCES

CPRG assay kit is designed to quantify low expression level β-Galactosidase.

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Amplite® Colorimetric Xanthine Oxidase Assay Kit

Amplite® Colorimetric Xanthine Oxidase Assay Kit

Supplier: AAT BIOQUEST

Xanthine oxidase (XO)is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyse the oxidation of xanthine to uric acid.

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