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VWR har reagenser og fargestoffer brukt innen mikroskopi for å fremheve og analysere spesifikke strukturer i celler eller vevsprøver.
Silver stain, FOCUS™ FASTsilver™
Supplier: G-Biosciences
FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.
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Gel loading dye, orange (6X)
Supplier: Thermo Fisher Scientific
Oange loading dye (6X) is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. Ideal for conventional DNA electrophoresis.
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VWR® EZ-Vision® Bluelight DNA Dye 10000X
Supplier: VWR Chemicals
EZ-Vision BlueLight is a sensitive, non mutagenic and environmentally safe fluorescent dye designed for gel staining.
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Immersion oil for microscopy, Sigma-Aldrich®
Supplier: Merck
Used to increase lens effectiveness
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Innstøpings- og monteringsmedier
Supplier: Merck
Den definerte hardheten og ensartetheten av materialet som skal kuttes, er svært viktige krav for å få en kutting av jevn kvalitet. Disse kravene kan oppfylles ved hjelp av en rekke egnede stoffer, for eksempel parafiner, plast, gelatin og celloidin.
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Immersion oil for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.
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Aqueous mounting medium, Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
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Xylene substitute, Neo-Clear®, Sigma-Aldrich®
Supplier: Merck
Neo-Clear® is a non hazardous alternative to xylene. It is virtually odourless, compatible with most high purity solvents and is characterised by lower evaporation rates than xylene, making it a lower health risk.
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Spurr low viscosity embedding kit, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Spurr resin is a hydrophobic resin. It is less viscous than epon mixtures and is easier to dispense. The low viscosity of the resin contributes to its penetration in tissue, mineral and dense structures. A low viscosity of 60 cps also allows easy penetration into a variety of difficult materials.
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Immersion oil viscosity 1,250 cSt (lit.), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Immersion oil has been used in epifluorescent microscopy and cell identification.
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HistoChoice® Clearing agent, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites and nucleic acid sites. This formulation in non-citrus, contains no mercury, formaldehyde or glutaraldehyde.
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Fikseringsmidler og reagenser for histologi
Supplier: Merck
M-FIX is a fixative spray designed for the wet fixation of gynaecological and non-gynaecological preparations processed in Papanicolaou staining. It contains polyglycols in alcoholic solution and a rapid and secure fixation of specimens is possible.
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Araldite® M, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.
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Embedding kit JB-4, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
JB-4 is a water-soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. It is a technique now widely used for research and clinical diagnosis. JB-4 yields semi-thin sections (0.5μ-2μ) with excellent morphological preservation.
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Epoxy embedding medium, hardener MNA, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator
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Hartman’s fixative histological tissue fixative, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination
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Aqueous mounting medium, Fluoroshield™ with PPD, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
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VWR® Q Path® Cytofix, Cytology Fixture
Supplier: VWR Chemicals
Fixative, combining the coagulant qualities of alcohol, to enable a better fixation of nuclear and cytoplasmic elements. The erythrocytes are lysed and, therefore, do not obscure observation of the elements of interest. This fixative can be used according to the Saccomanno method.
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VWR® Q Path® Freeze Gel (Glue) Mounting Medium for Cryotomy
Supplier: VWR Chemicals
High viscosity mounting media for cryotomy. Less viscous than O.C.T.
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IHC fluoromount, HIGHDEF®
Supplier: ENZO LIFE SCIENCES
Fluoromount is an aqueous-based permanent, high stability mounting medium designed for fluorogenic labelling and ideal for long-term storage of processed tissue.
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Antifade mounting medium
Supplier: ENZO LIFE SCIENCES
Antifade mounting medium (hardset) is an antifade reagent that causes little or no quenching. Use of the mounting medium significantly extends the photobleaching half-life of the fluorescent stain.
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Tissue-clearing reagent, CUBIC-HL for animals
Supplier: TCI
Specifications
Appearance: Colorless to Light yellow to Light orange clear liquid
Decoloring test: min. 1.0 (400 to 410 nm)
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Aqueous mounting medium, Fluoroshield™, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
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RenLam® M-1 for microscopy, suitable as a embedding medium, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
To be used with SIAL 69571 Ren HY 956 (Hardener for Epoxy Embedding Medium)
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Ren™ HY 956 epoxy embedding medium, hardener, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
to be used with SIAL 78711 RenLam M-1 (Epoxy Embedding Medium)
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Embedding medium, PolyFreeze, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.
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ImmunoHistoMount™ Mounting Medium
Supplier: SIGMA ALDRICH MICROSCOPY
ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.
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VWR®, OCT Embedding matrix for frozen sections
Supplier: VWR Chemicals
When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.
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Monteringsmedium, høyt, histokvalitet, J.T.Baker®
Supplier: Avantor
Monteringsmedium for dekkglass for slides.
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VWR® Q Path® O.C.T. Compound Mounting Medium for Cryotomy
Supplier: VWR Chemicals
High viscosity mounting medium for cryotomy.