You searched for: Protein Linking and Labeling Systems

Supplier: Thermo Fisher Scientific

The DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes. These dyes can be efficiently paired with many DyLight™ dyes and other common fluorescent dyes including fluorescein, rhodamine, cyanine and Texas Red® dye.

Supplier: AAT Bioquest

Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.

Supplier: AAT Bioquest

Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy5.

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with FITC.

Supplier: AAT Bioquest

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

Supplier: Thermo Fisher Scientific

Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

Supplier: AAT Bioquest

ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.

Supplier: AAT Bioquest

FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates.

Supplier: AAT Bioquest

XFD750, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 750, a trademark of ThermoFisher.

Supplier: AAT Bioquest

ReadiLink™ Protein Conjugation Stop Buffer is used to stop the protein conjugation reactions that use succinimidyl ester, sulfonyl chloride or isothiocyanate.

Supplier: AAT Bioquest

Cy5-Tris NTA compound is a sensitive fluorescent chelator for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces with the addition of certain metal ions (such as Ni²⁺, Co²⁺ etc.) as a mediator.

Supplier: Thermo Fisher Scientific

Pierce™ RNA 3' End Biotinylation Kit is optimised for labeling the 3' terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.

Supplier: Thermo Fisher Scientific

DyLight™ Dyes have absorption spectra ranging from 350 nm to 770 nm and match the output wavelengths of common fluorescence instrumentation.

Supplier: AAT Bioquest

XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher).

Supplier: AAT Bioquest

HIS Lite™ OG488-Tris NTA Chelator is used as a sensitive green fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion such as Ni²⁺ in cells, solution and solid surfaces.

Supplier: AAT Bioquest

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ rapid APC-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

Supplier: AAT Bioquest

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid APC-Cy5.5 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

Supplier: AAT Bioquest

The kit provides Taq DNA polymerase enzyme, which incorporates iFluor® 488-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).

Supplier: AAT Bioquest

Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.

Supplier: Antibodies.com

This 30 Minute IHC DAB Detection System is a powerful intensification system that significantly enhances chromogenic signals. This system can be integrated into standard immunohistochemistry (IHC) staining methods and results in a significant increase in sensitivity compared to standard IHC detection methods, while maintaining similar specificity.

Supplier: Thermo Fisher Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: AAT Bioquest

iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT Bioquest

iFluor® 555 PSA kit is a much superior replacement for Alexa Fluor 555 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT Bioquest

iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: Thermo Fisher Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Biotium

Mix-n-Stain™ Digoxigenin antibody labelling kits utilise a technology that allows rapid antibody labelling with digoxigenin without a purification step.

Supplier: Thermo Fisher Scientific

Maleimide-Activated Streptavidin is for directly preparing streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulphhydryl (-SH) group. Streptavidin has been maleimide-activated using Sulpho-SMCC, a heterobifunctional crosslinker that contains an N-hydroxysuccinimide ester and a maleimide group. The activated streptavidin presents an available maleimide group that reacts with sulphhydryl-containing molecules.

Supplier: Merck Millipore (Oncogene)

Biotin-NHS reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

Supplier: G-Biosciences

The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.