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You searched for: Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

Buccutite™ Poly-HRP Antibody Conjugation Kit

Buccutite™ Poly-HRP Antibody Conjugation Kit

Supplier: AAT Bioquest

Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level.

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Crosslink immunoprecipitation (IP) kit, Pierce™

Crosslink immunoprecipitation (IP) kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.

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Antibody labelling kits, DyLight™

Antibody labelling kits, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ Antibody Labeling Kits were specifically developed for fast, efficient labeling of antibodies. Two convenient kit formats are available to accommodate varied labeling requirements. The DyLight™ Antibody Labeling Kits contain all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins.

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Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional

Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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Speciality dyes, DyLight™

Speciality dyes, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for in vivo and near-IR fluorescence (NIRF) applications.

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NHS ester quenchers, DyLight™

NHS ester quenchers, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes. These dyes can be efficiently paired with many DyLight™ dyes and other common fluorescent dyes including fluorescein, rhodamine, cyanine and Texas Red® dye.

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Buccutite™ Rapid APC-Cy7 Tandem Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid APC-Cy7 Tandem Antibody Labelling Kits (2 Labellings)

Supplier: AAT Bioquest

APC-Cy7 is a popular colour used in flow cytometry.

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DY677 CEP (DyLight 677 Phosphoramidite)

DY677 CEP (DyLight 677 Phosphoramidite)

Supplier: AAT Bioquest

DY677 CEP (DyLight 677 phosphoramidite) is an excellent replacement to the commonly used Cy5.5 phosphoramidite.

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ReadiLink™ Rapid iFluor® Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid iFluor® Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT Bioquest

AAT Bioquest's iFluor® dyes are developed for labelling proteins, in particular, antibodies.

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Isobaric labelling reagent set, iodoTMTsixplex™

Isobaric labelling reagent set, iodoTMTsixplex™

Supplier: Thermo Fisher Scientific

The iodoTMTsixplex™ labelling reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry.

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Buccutite™ MTA, NHS ester  (2 µmoles)

Buccutite™ MTA, NHS ester (2 µmoles)

Supplier: AAT Bioquest

Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.

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Azide reactive biotinylation reagents, EZ-Link™ Phosphine-PEG3-Biotin

Azide reactive biotinylation reagents, EZ-Link™ Phosphine-PEG3-Biotin

Supplier: Thermo Fisher Scientific

EZ-Link® Phosphine-PEG3-Biotin is a versatile biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.

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ReadiLink™ Rapid XFD594 Antibody Labelling Kit

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).

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ReadiLink™ Rapid XFD488 Antibody Labelling Kit

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).

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ReadiLink™ Rapid iFluor® 350 Antibody Labelling Kit

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.

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Antibody biotinylation kit for IP, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Antibody Biotinylation Kit for IP provides biotinylation reagents designed specifically for the labelling of primary antibodies used in immunoprecipitation applications.

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FITC antibody labelling kit, Pierce™

FITC antibody labelling kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ FITC antibody labeling kit is a multi-component kit containing a high performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

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ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT Bioquest

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

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ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT Bioquest

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

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ReadiLink™ xtra Rapid Cy7 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

ReadiLink™ xtra Rapid Cy7 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

Supplier: AAT Bioquest

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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Wheat Germ Agglutinin, XFD647 Labelled *XFD647 Same Structure to Alexa Fluor® 647*

Wheat Germ Agglutinin, XFD647 Labelled *XFD647 Same Structure to Alexa Fluor® 647*

Supplier: AAT Bioquest

XFD647, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 647, a trademark of ThermoFisher.

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Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labelling Kits (2 Labellings)

Supplier: AAT Bioquest

APC-iFluor® 700 Tandem is an excellent replacement for APC-Alexa Fluor® 700 Tandem since they have almost identical spectra.

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HIS Lite™ iFluor® 647 Tris NTA-Ni Complex

HIS Lite™ iFluor® 647 Tris NTA-Ni Complex

Supplier: AAT Bioquest

iFluor 647-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.

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MitoROS™ 580 Kit for Detecting Reactive Oxygen Species (ROS) in Mitochondria

MitoROS™ 580 Kit for Detecting Reactive Oxygen Species (ROS) in Mitochondria

Supplier: AAT Bioquest

Oxidation of MitoROS™ 580 by superoxide generates red fluorescence.

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ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labelling Kit

Supplier: AAT Bioquest

Microscale optimised for labeling 50 µg antibody per reaction. ReadiLink™ Rapid mFluor™ Violet 610 antibody labelling kits provide a quick and convenient method to label antibodies, proteins (<10 kDa), or other amine-containing biomolecules.

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ReadiLink™ Rapid XFD488 Antibody Labelling Kit 'XFD488 Same Structure to Alexa Fluor™ 488' (2×50 µg Labellings)

ReadiLink™ Rapid XFD488 Antibody Labelling Kit 'XFD488 Same Structure to Alexa Fluor™ 488' (2×50 µg Labellings)

Supplier: AAT Bioquest

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

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HIS Lite™ iFluor® 568 Tris NTA Chelator

Supplier: AAT Bioquest

iFluor® 568 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion (such as Ni²⁺) in cells, solution and solid surfaces.

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ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)

ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)

Supplier: AAT Bioquest

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

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CytoWatch™ Wash-free Fluorescence Cell Imaging Buffer, 10X

CytoWatch™ Wash-free Fluorescence Cell Imaging Buffer, 10X

Supplier: AAT Bioquest

CytoWatch™ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimised for fluorescence cell imaging.

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Dye labeling kit (FITC), HOOK™

Supplier: G-Biosciences

FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.

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