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335 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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HIS Lite™ iFluor® 647 Tris NTA-Ni Complex

HIS Lite™ iFluor® 647 Tris NTA-Ni Complex

Supplier: AAT Bioquest

iFluor 647-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.

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MitoROS™ 580 Kit for Detecting Reactive Oxygen Species (ROS) in Mitochondria

MitoROS™ 580 Kit for Detecting Reactive Oxygen Species (ROS) in Mitochondria

Supplier: AAT Bioquest

Oxidation of MitoROS™ 580 by superoxide generates red fluorescence.

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ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labelling Kit

Supplier: AAT Bioquest

Microscale optimised for labeling 50 µg antibody per reaction. ReadiLink™ Rapid mFluor™ Violet 610 antibody labelling kits provide a quick and convenient method to label antibodies, proteins (<10 kDa), or other amine-containing biomolecules.

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ReadiLink™ Rapid XFD488 Antibody Labelling Kit 'XFD488 Same Structure to Alexa Fluor™ 488' (2×50 µg Labellings)

ReadiLink™ Rapid XFD488 Antibody Labelling Kit 'XFD488 Same Structure to Alexa Fluor™ 488' (2×50 µg Labellings)

Supplier: AAT Bioquest

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

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HIS Lite™ iFluor® 568 Tris NTA Chelator

Supplier: AAT Bioquest

iFluor® 568 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion (such as Ni²⁺) in cells, solution and solid surfaces.

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Buccutite™ Rapid PE-Cy5 Tandem Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid PE-Cy5 Tandem Antibody Labelling Kits (2 Labellings)

Supplier: AAT Bioquest

PE-Cy5 is a popular colour used in flow cytometry.

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ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)

ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)

Supplier: AAT Bioquest

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

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CytoWatch™ Wash-free Fluorescence Cell Imaging Buffer, 10X

CytoWatch™ Wash-free Fluorescence Cell Imaging Buffer, 10X

Supplier: AAT Bioquest

CytoWatch™ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimised for fluorescence cell imaging.

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Dye labeling kit (FITC), HOOK™

Supplier: G-Biosciences

FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.

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Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)

Supplier: AAT Bioquest

APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm.

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Buccutite™ Rapid PE Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid PE Antibody Labelling Kits (2 Labellings)

Supplier: AAT Bioquest

R-Phycoerythrin (PE) is an orange fluorescent protein which has an excitation wavelength of 565 nm and an emission wavelength of 575 nm.

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ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT Bioquest

AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.

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ReadiLink™ Rapid Cy7 Antibody Labelling Kit

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy7.

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ReadiLink™ Rapid Cy3 Antibody Labelling Kit

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy3.

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ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit

ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit

Supplier: AAT Bioquest

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Fluorescent dye removal columns, Pierce™

Fluorescent dye removal columns, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

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ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit

ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit

Supplier: AAT Bioquest

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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ReadiLink™Rapid mFluor™ Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™Rapid mFluor™ Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT Bioquest

mFluor™ dyes are developed for flow cytometry-focused applications.

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Carboxyl reactive biotinylation reagents, EZ-Link™

Carboxyl reactive biotinylation reagents, EZ-Link™

Supplier: Thermo Fisher Scientific

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.

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Helixyte™ iFluor® 594 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Helixyte™ iFluor® 594 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Supplier: AAT Bioquest

Helixyte™ iFluor® 594 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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Helixyte™ iFluor® 350 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Helixyte™ iFluor® 350 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Supplier: AAT Bioquest

Helixyte™ iFluor® 350 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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ReadiLink™ BSA Conjugation Kit (2 Reactions)

ReadiLink™ BSA Conjugation Kit (2 Reactions)

Supplier: AAT Bioquest

Bovine serum albumin (BSA) is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry.

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Pierce™ Deuterium-labelled Cross Linkers

Pierce™ Deuterium-labelled Cross Linkers

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

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Wheat Germ Agglutinin, XFD350 Labelled *XFD350 Same Structure to Alexa Fluor® 350*

Wheat Germ Agglutinin, XFD350 Labelled *XFD350 Same Structure to Alexa Fluor® 350*

Supplier: AAT Bioquest

XFD350, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 350, a trademark of ThermoFisher.

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ReadiLink™ xtra Rapid iFluor® 555 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

ReadiLink™ xtra Rapid iFluor® 555 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

Supplier: AAT Bioquest

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT Bioquest

iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

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Streptavidin, hydrazide-activated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Hydrazide-activated strepavidin conjugate includes recombinant streptavidin in a purified form activated for crosslinking to carbonyl groups in a molecule.

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Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers

Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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MS-Compatible magnetic IP kit (Protein A/G), Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.

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Pierce™ NHS-Fluorescein antibody labeling kit, Pierce™

Pierce™ NHS-Fluorescein antibody labeling kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ NHS-Fluorescein antibody labeling kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

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