Protein Extraction

Supplier: MP Biomedicals

Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.

Supplier: Thermo Fisher Scientific

Pierce™ High pH Reversed-Phase Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.

Supplier: Thermo Fisher Scientific

The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.

Supplier: Thermo Fisher Scientific

Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

Supplier: Thermo Fisher Scientific

The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Supplier: G-Biosciences

FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.

Supplier: Thermo Fisher Scientific

Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.

Supplier: Thermo Fisher Scientific

Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.

Supplier: Thermo Fisher Scientific

The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Supplier: Thermo Fisher Scientific

Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.

Supplier: Thermo Fisher Scientific

Imject™ Blue Carrier™ protein is a highly soluble, mollusc-derived hemocyanin that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.

Supplier: Thermo Fisher Scientific

Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.

Supplier: Thermo Fisher Scientific

Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Supplier: G-Biosciences

The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.

Supplier: G-Biosciences

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

Supplier: Thermo Fisher Scientific

The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.