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Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™

Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solutions for cell culture applications.

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Blocking buffers, Protein-Free™

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: G-Biosciences

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Tris EDTA buffer, for molecular biology, DNAse, RNAse, protease free ready to use, pH 8,0
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Permeabilization Buffer (Flow Cytometry), Abcam

Supplier: Abcam

Permeabilization Buffer (ab314681) is a ready to use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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Proteometer-L buffer packets

Proteometer-L buffer packets

Supplier: NOVILYTIC

7 sealed buffer packets specifically formulated for use with Proteometer-L kit.

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Extraction Enhancer Buffer 50X, Abcam

Supplier: Abcam

Abcam's Extraction Enhancer Buffer 50X is formulated for the preparation of cell or tissues extracts for use in immunoassays.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Rockland Immunochemicals

This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

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SSC buffer, 20X concentrate, pH 7,0

Supplier: Rockland Immunochemicals

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

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RIPA Buffer, Abcam

Supplier: Abcam

A ready-to-use buffer useful for lysing cells and tissues for radioimmunoprecipitation assay.

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Tissue Clearing Permeabilization Buffer - hydrophobic, Abcam

Supplier: Abcam

Tissue Clearing Permeabilization Buffer ab243307 is part of our range of tissue clearing reagents and products that enable you to set up tissue clearing easily and quickly.

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Proteometer-UFT buffer packets

Proteometer-UFT buffer packets

Supplier: NOVILYTIC

3 sealed buffer packets specifically formulated for use with Proteometer-UFT kit.

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Protein Block, Abcam

Supplier: Abcam

Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required

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GET buffer

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

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Buffer solution pH 5, Acetate Buffer

Supplier: Thermo Fisher Scientific

Acetate buffer, Contains 0.7 ml glacial acetic acid and 0.6 g potassium hydroxide per litre., pH value: 4,98 - 5,02 (20 °C)

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HeLa Nuclear Extract, Abcam

Supplier: Abcam

Nuclear extract is prepared from HeLa cells, which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.

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