Buffers

Supplier: Ambion

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Ambion

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Stemcell Technologies

RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.

Supplier: Ambion

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

Supplier: Molekula

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Stemcell Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

Supplier: Stemcell Technologies

Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.

Supplier: Molekula

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 7,5

Supplier: Stemcell Technologies

Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.

Supplier: Ambion

Running Buffer, TAE buffer solution 25X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Stemcell Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

Supplier: Molekula

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Stemcell Technologies

PBS buffer containing EDTA.

Supplier: Stemcell Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

Supplier: Stemcell Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.

Supplier: Stemcell Technologies

Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.