Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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VWR® AVS TITRINORM Buffer Solution, pH 9 (at 20 °C), Borate free

Supplier: VWR Chemicals

Solution prepared from AnalaR® NORMAPUR® grade analytical reagents.

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VWR®, Phosphate Buffered Saline (PBS), pH 7,4

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.

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VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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VWR®, Buffers, Sodium acetate

Supplier: VWR Chemicals

Sodium acetate buffer B, VWR®

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J.T.Baker®, Sheath fluid, 3000/3500

Supplier: Avantor

Sheath fluid 3000/3500, J.T.Baker®

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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Buffer, triethylammonium acetate, 1 M solution, pH 7,0

Supplier: PanReac AppliChem

Used for the purification of oligonucleotides.

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Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: PanReac AppliChem

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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10x Citrate Buffer pH 6.0, Abcam

Supplier: Abcam

Citrate buffer pH 6.0 for heat-induced antigen retrieval (HIER) during IHC.

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G-Biosciences Sodium Chloride-Sodium Phosphate-EDTA Buffers (20X)

Supplier: G-Biosciences

Saline-sodium phosphate-EDTA hybridisation buffer [20X].

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Non-animal protein blocking Agent, NAP-BLOCKER™

Supplier: G-Biosciences

NAP-BLOCKER™ is an animal-free protein blocking agent recommended for improved assay sensitivity, minimal non specific binding and a high signal-to-background ratio. It is suitable for Western blots, dot blots and assay development.