Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

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Native lysis Buffer, Abcam

Supplier: Abcam

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.

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100x Citrate Buffer pH 6.0, Abcam

Supplier: Abcam

Citrate buffer pH 6.0 for heat-induced antigen retrieval (HIER) during IHC (ab64236).

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Dry buffer packs, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.

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SDS-PAGE Sample Buffer, 2X, (2-Mercaptoethanol-free) for Electrophoresis

Supplier: TCI

SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.

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G-Biosciences Phosphate Buffered Saline, Sterile (1X and 10X)

Supplier: G-Biosciences

Ready-to-use, sterile, pH adjusted blend of phosphate buffer and saline solution.

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Buffer, PBS (10X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

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G-Biosciences Tris Buffered Saline, 10X

Supplier: G-Biosciences

TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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SSPE Buffer, 20X concentrate, pH 7,4

Supplier: Rockland Immunochemicals

20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.

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10X Tris-EDTA buffer pH 9,0

Supplier: Novus Biologicals

10X Tris-EDTA buffer pH 9.0.

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Buffer, TRIS solution pH 7 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 7 (1 mol/L)

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10X Phosphate Buffered Saline, Abcam

Supplier: Abcam

10X Phosphate Buffered Saline is a buffer designed for ELISA assay development.

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Cell Lysis Buffer, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 1067 Cell Lysis Buffer.

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Proteometer-UFT kit

Supplier: NOVILYTIC

The Proteometer-UFT Kit transforms any LC into an analytical system capable of identifying and quantifying titer content in crude culture filtrate in <90 seconds, without sample preparation.