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Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Proteometer-CV reconstitution reagent

Proteometer-CV reconstitution reagent

Supplier: NOVILYTIC

1 x vial of reconstitution reagent.

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SSPE Buffer, 20X concentrate, pH 7,4

Supplier: Rockland Immunochemicals

20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.

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10X RIPA Buffer, Abcam

Supplier: Abcam

10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins

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10X Tris-EDTA buffer pH 9,0

Supplier: Novus Biologicals

10X Tris-EDTA buffer pH 9.0.

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Proteometer-UFT kit

Proteometer-UFT kit

Supplier: NOVILYTIC

The Proteometer-UFT Kit transforms any LC into an analytical system capable of identifying and quantifying titer content in crude culture filtrate in <90 seconds, without sample preparation.

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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications (powder form).

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Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0), Abcam

Supplier: Abcam

Antigen retrieval 100X tris-EDTA buffer (pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. - Cited in over 20 publications - Contains 5% tris, 5% tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol - Optimized for tris-EDTA buffer (pH 9.0) treated antibodies - Contains detergent for paraffin emulsification

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Cell Lysis Buffer, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 1067 Cell Lysis Buffer.

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10X Phosphate Buffered Saline, Abcam

Supplier: Abcam

10X Phosphate Buffered Saline is a buffer designed for ELISA assay development.

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G-Biosciences MOPS Running Buffers (10X)

G-Biosciences MOPS Running Buffers (10X)

Supplier: G-Biosciences

A zwitterionic buffer providing consistent buffering capacity in the neutral range, ideal for RNA electrophoresis.

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G-Biosciences Red Blood Cell Lysis Buffer

Supplier: G-Biosciences

A red blood cell lysis buffer for the isolation of white blood cells free from interference of RBCs. The optimised concentration of ammonium chloride and enhances effectively lyses red blood cells, while preserving the integrity of the leukocytes.

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Protein blocking agent, BLOT-QuickBlocker™

Protein blocking agent, BLOT-QuickBlocker™

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

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Cell culture salts, salt solutions and buffers

Cell culture salts, salt solutions and buffers

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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Proteometer-CV consumables kit

Proteometer-CV consumables kit

Supplier: NOVILYTIC

The Proteometer-CV is designed for the scientific development of mAbs, bi-specifics, or fusion proteins with a human Fc region, regardless of their stage in the development continuum. This kit offers an alternative to traditional charge variant methods that require Protein A.

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Buffer, TRIS solution pH 8 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/L)

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Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

DNA wash buffer concentrate, low salt spin column was solution (makes 250 ml)

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