Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer concentrate, citrate phosphate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

These buffers are used to elute adult hemoglobin by the procedure of Oski and Naiman, a modification of the Kleihauer Betke procedure for the demonstration of fetal hemoglobin.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer solutions, according to EP and USP, Certipur®

Supplier: Merck

Buffer solutions intended for extremely precise calibration, qualification and monitoring of pH instruments, and for testing other buffer solutions.

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Standard reference materials and buffer solutions, CertiPUR®, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH instruments.

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Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH meters.

Sustainable Options Available

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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®

Supplier: Merck

1M Buffer, also for use in ion-exchange chromatography and electrophoresis.

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Phosphate buffer solution, BioUltra, Supelco®

Supplier: Merck

Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH value: 7,2

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TISAB solution for Fluoride Determination, Supelco®

Supplier: Merck

TISAB solution for fluoride determination

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Cesium chloride-aluminium nitrate buffer solution acc. to Schuhknecht and Schinkel, Supelco®

Supplier: Merck

Cesium chloride-aluminium nitrate buffer solution acc. to Schuhknecht and Schinkel for the determination of sodium, potassium and lithium by flame spectrometry (enth./cont. 50 g/l CsCl + 250 g/l Al(NO₃)₃ * 9 H₂O)

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Citrate buffer, pH 6.0, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Citrate buffer, antigen retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization.

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Tris-HCl buffer, pH 10, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.