An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.
Steady-Luc™ Firefly HTS assay kit (lyophilised)
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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ViaFluor® 488 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with green fluorescence.
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PROTEOSTAT® thermal shift stability assay kit
Supplier: ENZO LIFE SCIENCES
This kit has been designed for monitoring protein stability under systematic thermal stress conditions.
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Comet SCGE assay kit
Supplier: ENZO LIFE SCIENCES
COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.
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NETosis Assay Kit
Supplier: Cayman Chemical
For the measurement of NET-derived neutrophil elastase.
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α-Amylase Inhibitor Screening Kit
Supplier: Abcam
Simple, 1-step assay for screening potential α-amylase inhibitors.
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Calcium assay kit
Supplier: Cayman Chemical
For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.
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Potassium assay kits, FLIPR®
Supplier: MOLECULAR DEVICES
The FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z' values.
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Calcium assay kits, FLIPR® Calcium 4 assay kit
Supplier: MOLECULAR DEVICES
The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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p53 transcription factor assay kit
Supplier: Cayman Chemical
96-Well assay for measurement of p53.
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Steady-Luc™ Firefly HTS assay kit
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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Fluorometric HTS assay kits, Caspase-3 and Caspase-8
Supplier: Biotium
Caspase-3 and Caspase-8 Fluorometric HTS Assay Kits provide a homogenous assay system for fast and highly sensitive detection of Caspase-3 or Caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.
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Caspase-1 assay kit for drug discovery
Supplier: ENZO LIFE SCIENCES
This kit is a complete assay system to measure protease activity of recombinant caspase-1. Cleavage of a tetrapeptide substrate is monitored colorimetrically at 405 nm or fluorimetrically (Ex. 360 nm/Em. 460 nm).
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Caspase-3 fluorometric assay kit
Supplier: ENZO LIFE SCIENCES
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate DEVD-AFC.
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ROS Detection Cell-Based Assay Kit (DHE)
Supplier: Cayman Chemical
Measure superoxide and hydrogen peroxide levels in living cells.
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Caspase-2 fluorometric assay kit
Supplier: ENZO LIFE SCIENCES
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.
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Protein quantitation assay kits, CB-X™ Protein assay
Supplier: G-Biosciences
CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.
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Thermal shift protein stability kits, GloMelt™
Supplier: Biotium
Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.
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Glycogen Assay Kit
Supplier: Cayman Chemical
A simple and sensitive tool for assaying glycogen from tissue.
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PPARδ transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of PPARδ DNA binding activity.
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Reagents for Fluor-de-Lys®-Green HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
Cellular assay, Fluor-de-Lys®-green substrate, in 50 mM solution in DMSO
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Calcein-AM cell viability assay kits
Supplier: Biotium
Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.
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Apoptosis assay kit, NucView® 488 and MitoView™ 633
Supplier: Biotium
NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.
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ALDetect™ Lipid Peroxidation Assay kit
Supplier: ENZO LIFE SCIENCES
The assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid). It is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole with MDA and 4-hydroxyalkenals to yield a stable chromophore with maximal absorbance at 586 nm.
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Cellular senescence live cell analysis assay
Supplier: ENZO LIFE SCIENCES
Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.
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Free Fatty Acid Fluorometric Assay Kit
Supplier: Cayman Chemical
A sensitive tool for measuring free fatty acids in plasma, serum, and urine.
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FLUOR DE LYS® HDAC8 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.