You searched for: qPCR/RT-qPCR Enzymes and Kits

Supplier: Quantabio

Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.

Supplier: Thermo Fisher Scientific

The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

Supplier: Thermo Fisher Scientific

The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.

Supplier: VWR Chemicals

VWR® One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via dye-based qPCR. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

VWR® Probe One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via hydrolysis probes. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: Quantabio

PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Salmonella spp. in food samples. Kit that contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Salmonella spp.

Supplier: VWR Chemicals

VWR® Probe qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Legionella spp. in environmental samples (ISO/TS 12869). Kit contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Legionella spp.

Supplier: Quantabio

PerfeCTa® qPCR SuperMixes are 2X concentrated, ready to use reaction cocktails containing all components except primers, probe and template for qPCR.

Supplier: VWR Chemicals

VWR® Probe One-Step RT-qPCR Master Mix with UDG is designed for the detection and quantitation of RNA targets and is an ideal choice for multiplex applications. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

VWR® qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

This kit is designed to detect the pathogen microorganism Listeria monocytogenes in food samples, after enrichment steps, according to standard microbiology procedures. It can also be used to confirm suspect colonies isolated in selective culture media.

Supplier: Quantabio

FastMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility, and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. A key component of PerfeCTa® qPCR FastMixes is AccuFast™ Taq DNA polymerase that enables instant activation and rapid polymerisation kinetics. Upon heat activation (20 seconds at 95 °C), the antibodies irreversibly denature releasing fully active, unmodified Taq DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. FastMixes deliver maximum PCR efficiency, sensitivity, specificity, and a robust fluorescent signal with either fast or conventional cycling conditions.

Supplier: Quantabio

No compromises. Unparalleled performance meets sustainability.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.

Supplier: Quantabio

PerfeCTa® SYBR® Green SuperMixes and FastMixes™ enable efficient, sensitive and precise quantitative PCR with proprietary buffers and SYBR® Green stabilisers that maximise fluorescent signal, PCR efficiency, and reduce primer dimers. These SuperMixes and FastMixes™ have been optimised for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye or fluorescein.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Biotium

Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).

Supplier: Quantabio

These kits offer a convenient and highly sensitive solution for Real-Time reverse transcriptase PCR (qRT-PCR) of RNA templates using hybridisation probe detection chemistries, such as TaqMan® 5'-hydrolysis probes.

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

Supplier: Quantabio

Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.

Supplier: Quantabio

PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.

Supplier: Biotium

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

Supplier: Quantabio

qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.