You searched for: Nucleotides

Supplier: TriLink BioTechnologies

TriLink® N1-Methoxymethylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® 5-Methyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® Cytidine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

Supplier: TriLink BioTechnologies

A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-ethylpseudouridine can reduce the immunogenicity of the resulting mRNA.

Supplier: TriLink BioTechnologies

Biotin-16-Aminoallyl-2'-deoxyuridine can be enzymatically incorporated into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this NTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.

Supplier: ENZO LIFE SCIENCES

Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.

Supplier: TriLink BioTechnologies

TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

Supplier: ENZO LIFE SCIENCES

Bio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

Supplier: ENZO LIFE SCIENCES

Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

Supplier: ENZO LIFE SCIENCES

Cyanine 5-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP.

Supplier: TriLink BioTechnologies

TriLink® N1-Propylpseudouridine-5'-Triphosphate

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TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

Supplier: Biotium

Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mm.

Supplier: TriLink BioTechnologies

TriLink® 2'-O-Methylcytidine-5'-Triphosphate

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TriLink® 5-Propargylamino-2'-deoxycytidine-5'-Triphosphate

Supplier: AAT BIOQUEST

iFluor® 560-dUTP is a iFluor 560-modified nucleotide that can be used in various molecular biology applications, such as labelling and detection of DNA molecules.

Supplier: TriLink BioTechnologies

2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.

Supplier: ENZO LIFE SCIENCES

These ddUTPs are used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. ddUTP also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase.

Supplier: ENZO LIFE SCIENCES

Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

Supplier: Biotium

Highly purified mix of dNTPs (>99% by HPLC) in 0,6 mM Tris-HCl (pH 7,5); each dNTP is at a concentration of 10 or 25 mm in the solution provided.

Supplier: TriLink BioTechnologies

TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxythymidine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

This deoxyguanosine analog lacks the N7 nitrogen found in the natural guanine nucleobase. Replacement of this nitrogen atom with a carbon atom results in diminished structure of the major groove in DNA and may disrupt base stacking, but does not interfere with Watson-Crick base pairing. This is desirable for applications such as amplification and sequencing of GC-rich nucleic acid sequences.

Supplier: TriLink BioTechnologies

N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.).

Supplier: Cytiva

PCR nucleotide mix dNTP set (2 mM, 10 mM, or 25 mM each A,C,G,T) for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: TriLink BioTechnologies

TriLink® 2'-O-Methyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® Biotin-7-Deaza-7-Propargyl-dGTP

Supplier: TriLink BioTechnologies

TriLink® Uridine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA.