You searched for: Cell Culture Supplements and Additives

Supplier: ENZO LIFE SCIENCES

Accelerates drug optimisation with a unique collection of compounds of known safety and bioavailability for diverse targets.

Supplier: HyClone products (Cytiva)

SG-200 solution is a stable dipeptide of L-alanyl L-glutamine.

Supplier: HyClone products (Cytiva)

With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® ion channel ligand library contains 71 ion channel blockers and openers for use in characterising and identifying ion channels in individual cells or tissue.

Supplier: MP Biomedicals

M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E.coli and plasmid amplification.

Supplier: G-Biosciences

A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement used primarily for human cells and the production of cell secreted proteins. TCH™ is also effective in culturing other mammalian cells without the use of animal proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, recombinant insulin), which are heat treated.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Mammalian ProteaseArrest™ inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

Supplier: Biowest

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Supplier: PROVITRO

Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the tube. After usage store them in the dark at 2 °C to 8 °C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days.

Supplier: PROVITRO

The supplement kit for microvascular endothelial cell growth medium, advanced is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: PROTIDE PHARMACEUTICALS

When combined with DMSO, 2-8CELLsius™ can be utilised as a cryopreservation solution. 2-8CELLsius™ + DMSO is a cytoprotective, protein-free solution, with bio-stabilisers that support high post thaw cell viability. Optimal recovery is achieved through a novel approach to cell physiology at ultra-low temperatures.

Supplier: G-Biosciences

Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Supplier: HyClone products (Cytiva)

Dissociation reagents, such as trypsin, are used to detach and disaggregate adherent cells from the surface or substrate to which they are attached, as well as for dissociating tissues for primary cell culture.

Supplier: Biowest

Phenol red is used as a pH indicator in cell culture. Its colour exhibits a gradual transition from yellow to red over the pH range 6,8 to 8,2. Above pH 8,2, phenol red turns a bright pink (fuchsia) colour.

Supplier: G-Biosciences

The ProteaseArrest™ family of protease inhibitor cocktails are supplied as convenient, ready-to-use, liquid format cocktails that offer immediate >95% inhibition of harmful proteases. The 100X concentrated solution outperforms other similar protease inhibitor cocktails (including tablet formats) by offering instant inhibition, allowing delivery of optimised concentrations, demonstrating higher inhibition levels, and providing a greater ease of use. Also available as single use OneQuant™ ProteaseArrest™ aliquots.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Bacterial ProteaseArrest™ inhibits bacterial serine, cysteine and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Supplier: G-Biosciences

Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.

Supplier: G-Biosciences

ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

Supplier: Biowest

L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

Supplier: PROTIDE PHARMACEUTICALS

Ready to use, balanced, cytoprotective, protein-free solution with bio-stabilisers for short-term storage, regenerative medicine, stem cell processing and transportation of biological material. Short-term storage can be achieved at 2 to 8 °C or at ambient temperature for 24 to 48 hours. Does not contain any growth factors, hormones, or antibiotics. 2-8CELLsius™ can also be utilised as a cell washing solution.

Supplier: PROVITRO

The supplement kit for microvascular endothelial cell growth medium is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: Thermo Fisher Scientific

Halt protease and phosphatase inhibitor cocktail provides the convenience of a single solution with full sample protection. This broad-spectrum inhibitor cocktail is specifically optimised to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.

Supplier: PROTIDE PHARMACEUTICALS

A unique cryopreservation solution that does not contain DMSO or foetal bovine serum. Cellvation™ is convenient to use and provides optimal recovery. Cells can be exposed to Cellvation™ for longer periods of time before the freezing process is initiated. Cellvation™ is ready to use and can be used for cells cultured in serum-free medium or with serum-containing medium.

Supplier: PROVITRO

The Provitro skeletal muscle cell growth medium is a sterile liquid culture medium for culturing human skeletal muscle cells (HSKMC). The medium is delivered as a basal medium and is suitable for culturing Provitro HSKMC after adding the supplement kit components. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® ionotropic glutamatergic ligands plate contains 60 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The ionotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.