You searched for: Cell Culture Supplements and Additives

Supplier: HyClone products (Cytiva)

With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

Supplier: MP Biomedicals

M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E.coli and plasmid amplification.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® ion channel ligand library contains 71 ion channel blockers and openers for use in characterising and identifying ion channels in individual cells or tissue.

Supplier: G-Biosciences

A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: G-Biosciences

Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

Supplier: Biowest

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Supplier: G-Biosciences

ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

Supplier: PROVITRO

Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the tube. After usage store them in the dark at 2 °C to 8 °C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® histaminergic and melatonin ligands plate contains 41 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The Histaminergic and Melatonin Ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® hepatotoxicity library is a focused collection of 238 compounds with defined and diverse hepatotoxicity, including steatosis, mitochondrial toxicity, Mallory body formation, cholestatic effects, and many more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. The compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates. The library is an essential tool for predictive toxicology screening and assay development.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Bacterial ProteaseArrest™ inhibits bacterial serine, cysteine and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® ionotropic glutamatergic ligands plate contains 60 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The ionotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.

Supplier: G-Biosciences

Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.

Supplier: Biowest

L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

Supplier: G-Biosciences

The ProteaseArrest™ family of protease inhibitor cocktails are supplied as convenient, ready-to-use, liquid format cocktails that offer immediate >95% inhibition of harmful proteases. The 100X concentrated solution outperforms other similar protease inhibitor cocktails (including tablet formats) by offering instant inhibition, allowing delivery of optimised concentrations, demonstrating higher inhibition levels, and providing a greater ease of use. Also available as single use OneQuant™ ProteaseArrest™ aliquots.

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

Supplier: PROVITRO

The supplement kit for microvascular endothelial cell growth medium is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: Thermo Fisher Scientific

Halt protease and phosphatase inhibitor cocktail provides the convenience of a single solution with full sample protection. This broad-spectrum inhibitor cocktail is specifically optimised to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® opioid and sigma ligands plate contains 74 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The opioid and sigma ligands plate is 1 plate of the larger Neurotransmitter Library which contains over 650 ligands.

Supplier: ENZO LIFE SCIENCES

The GABAergic igands library contains 56 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The GABAergics ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 CNS receptor ligands.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® metabotropic glutamatergic ligands plate contains 49 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The metabotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® hematopoietic toxicity library is a focused collection of 115 compounds with defined and diverse hematopoietic toxicity, including myelosuppression, neutropenia, leukopenia, anaemia, and many more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. The compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates. The library is an essential tool for predictive toxicology screening and assay development.

Supplier: ENZO LIFE SCIENCES

The cholinergic ligands plate contains 72 compounds ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The cholinergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.

Supplier: THERMO FISHER DIAGNOSTICS

Egg yolk emulsion

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, salts, trace elements, and glucose. Manufactured to meet cGMP manufacturing standards and QC specifications.