You searched for: Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
ReadiUse™ Viral RNA Lysis Buffer
Supplier: AAT BIOQUEST
Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.
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I-PER™ Insect Cell Protein Extraction Reagents
Supplier: Thermo Fisher Scientific
The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
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Subcellular Protein Fractionation Kit for Cultured Cells
Supplier: Thermo Fisher Scientific
This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
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Protein extraction kit, bacterial PE LB™
Supplier: G-Biosciences
Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.
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Mammalian protein extract reagents, M-PER™
Supplier: Thermo Fisher Scientific
The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.
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ReadiUse™ Yeast Cell Lysis Buffer, 5X
Supplier: AAT BIOQUEST
ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.
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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)
Supplier: AAT BIOQUEST
Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
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Nuclear and cytoplasmic extraction reagents, NE-PER
Supplier: Thermo Fisher Scientific
The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.
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HEPES protein extraction buffer (5X)
Supplier: Thermo Fisher Scientific
Thermo Fisher Scientific
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Y-PER™ Yeast Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.
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Subcellular protein fractionation kit for tissues
Supplier: Thermo Fisher Scientific
This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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Protein extraction kit, Insect PE LB™
Supplier: G-Biosciences
Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.
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Protein extraction reagent, BugBuster® 10X, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.
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RBC lysis buffer for human
Supplier: Thermo Fisher Scientific
RBC lysis buffer for human, Thermo Fisher Scientific
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T-PER™ Tissue Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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CHAPS lysis buffer
Supplier: Thermo Fisher Scientific
For cell lysis and protein extraction.
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Denaturing lysis buffer
Supplier: Thermo Fisher Scientific
Denaturing lysis buffer, Thermo Fisher Scientific
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RBC lysis buffer for mouse
Supplier: Thermo Fisher Scientific
RBC lysis buffer for mouse, Thermo Fisher Scientific
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HEPES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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NP-40 lysis buffer
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, Thermo Fisher Scientific
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Cell lysis buffers
Supplier: Rockland Immunochemicals
Cell lysis buffer is used to lyse cells under non-denaturing conditions.
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NP-40 lysis buffer with glycerol (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific
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Triton® X-100 lysis buffer, pH 7,4
Supplier: Thermo Fisher Scientific
Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific
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MNE buffer, pH 6,5
Supplier: Thermo Fisher Scientific
MNE buffer, pH 6.5, Thermo Fisher Scientific
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NP-40 lysis buffer, low salt
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, low salt, Thermo Fisher Scientific
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HEPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific