Biological Buffers

Biological buffers are inert and have short-term effects on the system under investigation, both of which are distinct advantage over inorganic substances and weak organic acids that were used in the past. Most biological buffers in use today are N-substituted taurine or glycine buffers, which meet most of the requirements that biological buffers have to fulfill. Buffer systems are usually used for experiments to enable direct comparison of results.

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Phosphate Buffer Solution

Supplier: SIGMA ALDRICH MICROSCOPY

Contains sodium and potassium phosphates. Intended for use in the Sigma Butyrate Esterase Procedure, No. 181B.

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Trizmal™ Buffer, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

For use in the Naphthol AS-D Chloroacetate Esterase Kit No. 91-C.

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Citrate Buffer, Concentrate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Citrate buffer solution, 0.38 mol/L, pH 5.4 when diluted according to procedure 386A.

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Tris-EDTA (TE) buffer solution for molecular biology, pH 7,4, BioUltra, Supelco®

Supplier: Merck

Tris-EDTA buffer solution has been used for the dilution of DNA samples.[1] It has been used for the elution of purified vector.

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Buffer concentrate, citrate phosphate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

These buffers are used to elute adult hemoglobin by the procedure of Oski and Naiman, a modification of the Kleihauer Betke procedure for the demonstration of fetal hemoglobin.

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VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology

Supplier: VWR Chemicals

Sodium acetate buffer, VWR®, 3 M, pH value: 5,2

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Phosphate buffer solution, BioUltra, Supelco®

Supplier: Merck

Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH value: 7,2

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J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT is a ready-to-use dissolution media concentrate, which is pre-measured and only need to be diluted with purified water.

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RNA isolation buffers

Supplier: OMEGA BIO-TEK

TRK lysis buffer

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Buffer kit for calibration of fluorescent Ca² indicators

Supplier: Biotium

Kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca² indicators (1,2).

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Optimizer Buffer™ for optimal cross-linking and modification

Supplier: G-Biosciences

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.