219 Results for: "Sodium bicarbonate "

Supplier: Biowest

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

Supplier: HyClone products (Cytiva)

HyClone™ SFX-Insect™ with L-glutamine is a versatile protein-free cell culture medium developed through metabolic pathway design to support the growth of multiple insect cell lines and production of a variety of recombinant proteins. It provides superior growth of many key insect cell lines, including Sf9, Sf21, and High Five (T.ni), requiring minimal adaptation.

Supplier: Biowest

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Supplier: Biowest

The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Supplier: Biowest

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Supplier: Biowest

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

Supplier: HyClone products (Cytiva)

Ham's F-10 was designed for use with Chinese Hamster Ovary (CHO) cells, and has been shown to support the growth of human diploid cells, white blood cells, and primary explants from rat, rabbit, or chicken tissues. This classical media can be used for serum-free growth of some cell lines including CHO cells. Serum supplementation may or may not be necessary depending on the type of cell being cultured. Ham's F-10 contains a wide variety of components, including zinc, hypoxanthine, and thymidine and is buffered with sodium bicarbonate.

Supplier: Biowest

Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

Supplier: MP Biomedicals

Dulbecco's Modified Eagle's Medium (DMEM) with L-Glutamine with 4,5 g/L glucose without sodium bicarbonate is the most widely used modification of Eagle's Basal Medium (BME).

Supplier: Honeywell Chemicals

Certified Reference Material (CRM).

Supplier: Honeywell Chemicals

Certified Reference Material (CRM).

Supplier: MP Biomedicals

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers.

Supplier: Serva

Sodium hydrogen carbonate

Supplier: Serva

Sodium hydrogen carbonate

Supplier: MP Biomedicals

Dulbecco's Modification of Eagle's Medium with 20 mM HEPES and 4,5 g Dextrose/liter, without L-glutamine or Sodium bicarbonate is ideally suited for supporting and maintaining a vast range of mammalian cell types.

Supplier: Corning

A complete range of classical cell culture media are available.

Supplier: Thermo Fisher Scientific

Sodium bicarbonate buffer solution, 1,0 M, pH value: 9,4, Liquid

Supplier: Hach

Natural Water Standard Solution:40% sodium sulphate/40% sodium bicarbonate/20% sodium chloride.

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

Supplier: G-Biosciences

Sodium hydrogen carbonate

Supplier: Corning

0,05% Trypsin/0,53 mM EDTA in HBSS (Hank’s Balanced Salt Solution) with sodium bicarbonate, without calcium and magnesium.

Supplier: MERCK PRODUCTION CHEMICALS

Sodium hydrogen carbonate FCC, E500, SAFC®

Supplier: Corning

Corning®, Iscove’s Modified DMEM: with L-glutamine and 25 mM HEPES, without [beta]-thioglycerol, [beta]-mercaptoethanol, and sodium bicarbonate, Powder

Supplier: MP Biomedicals

Minimum Essential Medium Eagle With Earle's Salts and 2,0 g/L sodium bicarbonate Without L-glutamine, L-cysteine, L-cystine, and L-methionine is generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Supplier: Honeywell Chemicals

Sodium hydrogen carbonate ≥99.7% ACS, Fluka™

Supplier: HyClone products (Cytiva)

Buffer cell culture with a fully QC-tested solution.

Supplier: ACTIVE MOTIF

Sodium hydrogen carbonate 0.5 mol/l in aqueous solution

Supplier: MP Biomedicals

Soluble in water (100 mg/ml).