2754 Results for: "STEMCELL Technologies"

Supplier: STEMCELL Technologies

Density gradient medium for the isolation of viruses, organelles, macromolecules, or cells.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b, antibody against human, chimpanzee, CD45RA, APC-Cyanine7-conjugated.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched mouse epithelial cells

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched non-human primate T cells

Supplier: STEMCELL Technologies

Immunomagnetic cell isolation kit using particle release technology.

Supplier: STEMCELL Technologies

Stimulating T cells with HHV8 (K8) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV8 (K8) peptide pool is a lyophilised mixture of 57 peptides from protein K8 alpha of human herpesvirus 8 (HHV-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 237 on K8. A basic leucine zipper (bZip) protein that associates with the lytic origin of HHV8 (Lin et al.), K8 is thought to play a role in lytic DNA replication, as a trans-acting factor (AuCoin et al.). It has also been found to modulate the activity of the transcriptional transactivator K-Rta (Izumiya et al.), and has an autoregulatory function (Wang et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with JCV (LT) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. This peptide pool is a lyophilised mixture of 170 peptides from large T antigen (LT) of JC polyomavirus (JCV), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 688 on LT. LT is detectable during JCV latency (Rossi et al.), and in immunocompromised patients with JVC-associated pathologies such as progressive multifocal leukoencephalopathy (PML) (Delbue et al.). In PML, LT has been associated with activating the promoter of survivin, an anti-apoptotic protein (Del Valle et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD138 (syndecan-1), APC-conjugated.

Supplier: STEMCELL Technologies

Complete kit for hematopoietic CFU assays.

Supplier: STEMCELL Technologies

Supplement (50X) for the serum-free culture of neurons.

Supplier: STEMCELL Technologies

Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation of myeloid hematopoietic progenitor cells to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts. M-CSF is a crucial factor for the development of tissue-resident macrophages in most tissues (Ginhoux and Jung). It is required for the maturation and activation of monocytes and macrophages, and regulates inflammatory responses in conjunction with other stimuli such as IFN-γ, LPS, and IL-4 (Murray et al.). M-CSF is also required for bone resorption by osteoclasts, and is involved in the development and regulation of the placenta, mammary gland, and brain. M-CSF is produced by monocytes, fibroblasts, osteoclasts, stromal cells, endothelial cells, and tumor cells (Chockalingam and Ghosh). M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase (CSF-1R or M-CSF-R) encoded by the c-fms proto-oncogene (Hamilton). CSF-1R shares similar structural features with other growth factor receptors, including the stem cell factor (SCF) receptor, platelet-derived growth factor receptor (PDGF-R), and Flt3/Flk-2 receptor tyrosine kinase. Stimulation of the CSF-1R upon binding to M-CSF activates MAPK, PI3K, and PLCγ signaling pathways (Chockalingam and Ghosh). Human and mouse M-CSF sequences are highly conserved both at nucleotide and amino acid levels (80% homology; DeLamarter et al.).

Supplier: STEMCELL Technologies

TRAIL (TNF-related apoptosis-inducing ligand) belongs to the tumor necrosis factor (TNF) superfamily and is associated with initiating apoptosis. TRAIL has four major receptors: two death receptors DR4 and DR5, and two decoy receptors DcR1 and DcR2. TRAIL binds to the death receptors, which recruits the Fas-associated death domain (FADD) and activates caspases 8 and 10, which eventually leads to apoptosis (Pitti et al.; Wiley et al.; Zauli and Secchiero). It has been shown that mice lacking the expression of TRAIL have defects in thymocyte apoptosis and negative selection, and these mice had increased susceptibility to autoimmune diseases (Lamhamedi-Cherradi et al.).

Supplier: STEMCELL Technologies

Insulin-like growth factor 1 (IGF-I) is a polypeptide that belongs to the family of insulin-like growth factors that are similar in molecular structure to proinsulin. IGF-I binds to the IGF-I receptor and is a potent activator of the PI3K/AKT pathway and also activates ERK1/2 signaling. IGF-I is required for embryonic development, and it is produced mainly in the liver in response to a hepatocyte growth hormone. In the absence of insulin, IGF-I is necessary for the maintenance of human pluripotent stem cells (Wang et al.). Together with IL-3, IGF-I stimulates differentiation and proliferation of myeloid cells and has been shown to regulate lymphopoiesis by stimulating proliferation and differentiation of T and B cells in lymphoid organs (Heemskerk et al.).

Supplier: STEMCELL Technologies

Culture medium kit for initiation, growth, and differentiation of human liver organoids.

Supplier: STEMCELL Technologies

Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells.

Supplier: STEMCELL Technologies

Amine-labelling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

Supplier: STEMCELL Technologies

R-Spondin-1 (RSPO1) is the prototype member of the R-Spondin (RSPO) protein subfamily of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins (Chen et al.; Kamata et al.; Kazanskaya et al.; Kim et al.). Although unable to initialize signaling, RSPO family members are potent enhancers of WNT signaling (Cruciat and Niehrs; de Lau et al.; Kamata et al.; Kazanskaya et al.). They are characterized by a TSR-1 domain, a carboxy-terminal region with positively charged amino acids, and two N-terminal furin-like cysteine-rich repeats (Glinka et al.; Kazanskaya et al.). R­-Spondin-1 activates β­-catenin signaling via the WNT signaling cascade and by indirectly increasing low-density lipoprotein receptor-related protein 6 (LRP6) on the cell surface. It does this by binding leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and competing with WNT antagonist DKK­1 for binding to the WNT co­receptors, Kremen and LRP­6, which reduces DKK­1-­mediated internalization of LRP6 (Binnerts et al.). RSPO1 is involved in a wide range of pleiotropic roles during embryogenesis, it is required for the specification of hematopoietic stem cells, and it has been shown to be important in the growth, survival, and migration of ovarian cancer cells (Cruciat and Niehrs; de Lau et al.; Genthe and Clements; Liu et al.).

Supplier: STEMCELL Technologies

Vascular endothelial growth factor (VEGF-121) is a naturally-occurring VEGF-A splice variant involved in embryonic vasculogenesis and angiogenesis. VEGF binds to VEGFR-1 and VEGFR-2, and activates Raf/MEK/ERK and PI3K/AKT pathways. VEGF-121 is released as a freely diffusible protein by a variety of normal and transformed cells. It plays an important role in neurogenesis both in vitro and in vivo (Storkebaum et al.). It has neurotrophic effects on neurons of the central nervous system and promotes growth and survival of dopaminergic neurons and astrocytes. VEGF also promotes growth and survival of vascular endothelial cells, monocyte chemotaxis, and colony formation by granulocyte-macrophage progenitor cells (Ferrara et al.).

Supplier: STEMCELL Technologies

Myeloma and hybridoma culture medium (serum-containing).

Supplier: STEMCELL Technologies

Stimulating T cells with SARS-CoV-2 (Spike Protein) Omicron/B.1.1.529 Mutation Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. A lyophilised mixture of 80 peptides of the spike glycoprotein of SARS-CoV-2 Omicron variant (B.1.1.529), these peptides selectively cover the mutated regions in the spike protein of the SARS-CoV-2 Omicron variant, compared to wild-type. Through interactions with the angiotensin-converting enzyme 2 (ACE2) receptor, the spike protein enables the virus to attach to the cell membrane, and plays a critical role in viral entry (Hoffmann et al.; Walls et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

The principal physiological regulator of erythropoiesis, Erythropoietin (EPO) is a glycoprotein growth factor that is produced primarily in the kidney in response to hypoxia or anemia. EPO promotes erythropoiesis by binding to a homodimeric cell surface receptor that activates JAK2/STAT5, PI3K/AKT, and MAPK pathways, and stimulates the proliferation and differentiation of erythroid progenitor cells (Jelkmann. Transfus Med Hemother, 2013; Kuhrt and Wojchowski. Blood, 2015).

Supplier: STEMCELL Technologies

Complete kit for optimisation of genome editing in human cells.

Supplier: STEMCELL Technologies

cGMP, stabilised feeder-free maintenance medium for human ES and iPS cells.

Supplier: STEMCELL Technologies

Influenza (HLA class I control) peptide pool is a lyophilised mixture of 17 peptides from the influenza virus. The pool consists of defined HLA class I-restricted T cell epitopes, and can be used as an antigen-specific positive control to stimulate T cells.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD4, FITC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83, APC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD90 (Thy-1), PerCP-Cy5.5-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgM antibody against human, rhesus, rabbit TRA­1­60 (podocalyxin), Alexa Fluor® 488-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b antibody against human, mouse, rat GFAP (glial fibrillary acidic protein), unconjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG3, kappa isotype control antibody, Alexa Fluor® 488-conjugated.