Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Supplier: Merck Millipore (Novagen)

D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.

Supplier: Merck Millipore (Novagen)

The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

Supplier: Merck Millipore (Novagen)

The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation.

Supplier: Merck Millipore (Novagen)

The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems.

Supplier: Merck Millipore (Novagen)

BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Supplier: Merck Millipore (Novagen)

Convenient solution for selective degradation of RNA.

Supplier: Merck Millipore (Novagen)

S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

Supplier: Merck Millipore (Novagen)

ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.

Supplier: Merck Millipore (Novagen)

NanoJuice™ Core transfection reagent is a polycationic liposomal which is designed to be used with the NanoJuice™ Transfection Booster. It is highly flammable.

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

Supplier: Merck Millipore (Novagen)

Molecular size markers for routine use in plasmid cloning, the Perfect DNA™ markers contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities except for the indicated reference bands, which are useful for instant band identification.

Supplier: Merck Millipore (Novagen)

Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.

Supplier: Merck Millipore (Novagen)

pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

Proteinase K

Supplier: Merck Millipore (Novagen)

Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

Supplier: Merck Millipore (Novagen)

The NovaTaq PCR Master Mix is a ready to use 2X concentrated mixture of NovaTaq DNA polymerase, ultra-pure deoxynucleotides, and reaction buffer without magnesium chloride. The master mix simplifies the assembly of PCR reactions and offers advantages of times savings, consistency, and minimal risk of contamination. Simply add the NovaTaq PCR master mix to an equal volume containing the required amount of magnesium chloride, DNA template, and primers, and the reaction is ready for thermal cycling. The final diluted reaction contains 2,5 units of NovaTaq DNA polymerase per 100 µl. Sufficient components are included for 200 standard 50 µl (or 100×100 µl) amplification reactions.

Supplier: Merck Millipore (Novagen)

KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.

Supplier: Merck Millipore (Novagen)

RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.

Supplier: Merck Millipore (Novagen)

rLysozyme™ solution contains a highly purified and stabilised recombinant lysozyme that can be used for lysis of gram negative bacteria, such as E. coli.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

Supplier: Merck Millipore (Novagen)

The Novagen® BacMagic™-2 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

Supplier: Merck Millipore (Novagen)

Veggie yeast extract is a certified animal-free media component.

Supplier: Merck Millipore (Novagen)

NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and recA and endA mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.