"EDVOTEK"
Blue light transilluminator, TruBlu™
Supplier: EDVOTEK
The TruBlu™ blue light transilluminator is ideal for viewing DNA gels stained with SYBR® Safe, thus eliminating the need for UV light or ethidium bromide. It is optimised to fit Edvotek® gels as well as any other agarose gels.
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Graduated pipettes, disposable
Supplier: EDVOTEK
Pipette, graduated, Plastic, 5 ml
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Safety spectacles, Laboratory
Supplier: EDVOTEK
Practical, durable safety spectacles offering good protection and comfort.
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SYBR® Safe Stain
Supplier: EDVOTEK
SYBR® Safe Stain is a DNA stain that fluoresces with a bright green colour when excited with UV light. Like ethidium bromide, SYBR® Safe Stain binds specifically to the DNA double helix.
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Floating racks
Supplier: EDVOTEK
Floating rack is ideal for floating tubes in water baths and tubs.
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Experimentation Kits, DNA Fingerprinting Using Restriction Enzymes
Supplier: EDVOTEK
DNA fingerprinting are featured in this lab by comparing crime scene DNA with suspect DNAs. Fingerprint patterns are separated by agarose gel electrophoresis.
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Experimentation Kit, Blood Typing for Stem Cell Transplants
Supplier: EDVOTEK
In this experiment, students will take on the role of transplant physicians. They will analyze simulated blood samples for transplant compatibility and compare genetic markers to identify which donor is the best match for a leukemia patient in need of a stem cell transplant.
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Experimentation kits, cleavage of Lambda DNA with Eco RI endonuclease: intro to restriction enzymes
Supplier: EDVOTEK
The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for Eco RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.
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Transformation of E. coli with pGAL™
Supplier: EDVOTEK
In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.
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Transformation with blue and green fluorescent proteins
Supplier: EDVOTEK
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.
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Transformation with green fluorescent protein
Supplier: EDVOTEK
In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.
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Experimentation kits, DNA fingerprinting using restriction enzymes
Supplier: EDVOTEK
This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.
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Kits: restriction enzyme cleavage patterns of DNA, Ready-to-Load™
Supplier: EDVOTEK
Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.
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Ready-to-Load™ Kits, PCR
Supplier: EDVOTEK
Ready-to-Load™ PCR kit introduces students to the principles and applications of the Polymerase Chain Reaction (PCR). This simulation experiment does not contain human DNA and does not require a thermal cycler.
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Kits: mapping of restriction sites on plasmid DNA, Ready-to-Load™
Supplier: EDVOTEK
DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.
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