Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

Supplier: MP Biomedicals

Applications
Guanidine hydrochloride can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA. It is a strong chaotropic agent useful for the denaturation and subsequent refolding of protein, it can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA.
Product Description
The crystal structure of Guanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds,it is a strong chaotropic agent.Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a freeflowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical

Grade: Ultra Pure
Purity: >99%
Keywords: Guanidium chloride, chaotropic agent
Key Applications: Chaeotropic agent
Product Type: Biochemicals
Biochemical Category: Chaotropic Agents
Density: 1.345 g/cm³ at 20 °C (Lit.)
Melting Point: 180-190 °C
UV/Visible Absorbance: OD260nm (6.0 M aq soln) <0.03
Presentation: White Crystalline Powder
pH: 4-6 (6.0 M aq soln)
Solubility: Soluble in water,Clear, Colorless Solution (6 M - clear, colorless solution).
Storage & Handling: Room Temperature, desiccate

Supplier: OMEGA BIO-TEK

Isolate DNA from up to 10 ml blood using magnetic beads.

Supplier: Thermo Fisher Scientific

Rigid PC frame, thin-walled PP wells. Ultra-rigid 96-well PCR plates with rounded V-bottom wells designed for robotic applications.

Supplier: Thermo Scientific

Thermo Scientific™ TDE Series ultra-low temperature freezers, powered by H-drive, feature four upright models, maximizing storage capacity from 30000 up to 60000 2 ml vials. The TDE Series is a sustainable and reliable solution with ultimate sample security and operational savings for every laboratory. Sustainability features include natural refrigerants and water-blown foam, compliant with many sustainability standards.

Supplier: VITLAB

Volumetric flask, PMP, class B, with screw cap, PP, 250 mL 1 * 2 items

Supplier: witeg Labortechnik

FLASK,PEARSHAPED,25ML,SIDE NECK ANGLED,CNST19/26,SNST19/26,ACC.TO DIN12383 1 * 1 items

Supplier: CONSORT

REDOX STANDARD SOLUTION 500 ML 124 MV 1 * 500 mL

Supplier: Hach

Bottle Cap, with inert Teflon® liner for mixing bottle, pk/12 1 * 12 items

Supplier: STEMCELL Technologies

Interleukin 31 (IL-31), a four-helix bundle inflammatory cytokine, belongs to the IL-6 cytokine family which includes IL-6, oncostatin M, leukemia inhibitory factor (LIF), and cardiotrophin-1 ( Dillon et al.). IL-31 signals through a heterodimer composed of IL-31RA (also known as gp130-like receptor or GPL) and the oncostatin-M receptor (OSMR), both of which are expressed on monocytes (Diveu et al.; Ghilardi et al.), epithelial cells (Ip et al.), and keratinocytes (Kato et al.). Signaling through the GPL/OSMR complex activates the JAK/STAT, RAS/ERK, and PI3K/AKT signaling pathways, resulting in the downstream activation of STAT1, STAT3, and STAT5 transcription factors (Cornelissen et al.; Dambacher et al.; Dillon et al.; Ip et al.). IL-31 responses have been associated with allergic responses and inflammatory skin diseases including atopic dermatitis (Cornelissen et al.; Gangemi et al.).

Supplier: VITLAB

Volumetric flask, PMP, class B, with screw cap, PP, 500 mL 1 * 2 items

Supplier: BUCHI

Semi-automated vacuum controller V-850 with a 1/4 VGA LCD-display to control the vacuum by a valve or by the new speed control techn ology with the Vacuum Pump V-700/V-710. The cont 1 * 1 items

Supplier: LAUDA

The Proline Edition X range of heating thermostats feature a wide temperature range, high cooling and heating power and a multi level pump. The self-explanatory menu-guided remote control Command is particularly convenient for operating the units at distances as far as 50 meters away. Thanks to the large display, the user can keep an eye on all the important parameters. There is a graphical display of measured values, large choice of interfaces (e.g. Ether- CAT, Ethernet, RS232/RS485, analogue module, Profibus, contact modules). Data collection together with documentation and control is easy using the Wintherm Plus software. Units are ideal for temperature control of chemical reactors, distillation plants and glass condensers, crystallisation, thermostating measurement setups in process technology, chemical probes, calibration of sensors and testing electronic components.

Supplier: Brand

PP. These 0,2 and 0,5 ml PCR tubes with attached caps are compatible with leading thermal cyclers with heated lids.

Supplier: MP Biomedicals

Nucleic Acid Purification Kits and Reagents, PPS solution

Supplier: VWR Collection

The housing and tank are constructed from high-quality SUS 304 stainless steel, providing durability and longevity. Equipped with user-friendly interface display which offers intuitive function selection and real-time parameter setting. Units also feature intelligent detection on startup, promptly alerting users to any potential issues via buzzer and alarm. Additionally, they continuously monitor the power supply voltage in real-time to ensure optimal operating conditions. The automatic heating stirring function ensures uniform liquid temperature throughout the tank. The anti-leakage tray prevents damage to electrical components and avoids the risk of hazard in case of leakage.

Supplier: Sartorius Balances

The Cubis® II laboratory balances are modular, therefore they allow to choose between applications and configurations which suit the best to the needs. These balances can be configured at the level of display, draftshields, software applications and hardware functions.

Supplier: TRANE TECHNOLOGIES

Purpose-built for life science applications, FARRAR®’s ULC series including the Ultra-Low Chamber (ULC-190), Ultra-Low Chamber (ULC-328), and the new Ultra-Low Chamber (ULC-259) are the only −20 to −80 °C, forced-air, ultra-low-temperature freezers on the market with air and water-cooled options.

Supplier: Thermo Fisher Scientific

D-(+)-Camphor ≥98%

Supplier: KIMBERLY CLARK

GLOVE STERLING NITR. 24CM AMBI GREY XL 1 * 140 items

Supplier: MACHEREY-NAGEL

Filter Paper Circles MN 85/70 20 cm diameter pack of 100 1 * 100 items

Supplier: Sartorius Balances

The Cubis® II laboratory balances are modular, therefore they allow to choose between applications and configurations which suit the best to the needs. These balances can be configured at the level of display, draftshields, software applications and hardware functions.

Supplier: Sartorius Balances

The Cubis® II laboratory balances are modular, therefore they allow to choose between applications and configurations which suit the best to the needs. These balances can be configured at the level of display, draftshields, software applications and hardware functions.

Supplier: Sartorius Balances

The Cubis® II laboratory balances are modular, therefore they allow to choose between applications and configurations which suit the best to the needs. These balances can be configured at the level of display, draftshields, software applications and hardware functions.

Supplier: TCI

D-(+)-Camphor ≥98.0% (by GC)

Supplier: STEMCELL Technologies

Interleukin 17A (IL-17A) is the founding member of the family of cytokines that includes IL-17B through IL-17F. It is a potent pro-inflammatory cytokine that plays a key role in defense against pathogens. IL-17A and IL-17F signal as homodimers or heterodimers through the same receptor, and activate NF-κB, MAPK, and C/EBP pathways (Gaffen). IL-17A is produced by Th17 cells, CD8+ T cells, γ/δ T cells, natural killer (NK) T cells, B cells, innate lymphoid cells, and mesenchymal stromal cells (MSCs) (Cua and Tato; Gaffen; Mojsilović et al.). IL-17A mediates protection against extracellular pathogens, and together with IL-22 stimulates production of antimicrobial peptides. It induces granulopoiesis factors and neutrophil-specific chemokines. Together with tumor necrosis factor alpha (TNF-α), IL-17A induces a sustained neutrophil recruitment during inflammation (Cua and Tato). IL-17A receptor is expressed at particularly high levels on stromal cells, including MSCs. IL-17A increases the frequency and the average size of fibroblast colony-forming units (CFU-F), as well as the proliferation of marrow-derived MSCs. It enhances osteogenic differentiation, and inhibits adipocyte differentiation and chondrogenesis (Mojsilović et al.).

Supplier: STEMCELL Technologies

Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine that regulates a broad variety of developmental functions. After LIF binds to LIF receptor (LIFR), LIFR associates with gp130 and activates JAK/STAT and MAPK signaling (Auernhammer and Melmed; Suman et al.). LIFR activation of STAT3 is essential for maintaining the mouse embryonic stem cell phenotype (Niwa et al.). Produced by the endometrium, LIF plays an important autocrine and paracrine role in implantation by regulating proliferation, invasion, and differentiation of trophoblasts following blastocyst attachment (Auernhammer and Melmed; Suman et al.). Human LIF can be used for the maintenance of mouse embryonic stem cells, however mouse LIF cannot bind to the human receptor, thus rendering mouse LIF inactive (Dahéron et al.). LIF is produced by CD4+ and activated regulatory T cells, and promotes Foxp3 expression, while repressing Th17 lineage-specific genes (Metcalfe). LIF is also secreted by mesenchymal stromal cells, where it supports hematopoiesis and immune modulation (Nasef et al.). This product is animal component-free.

Supplier: Brand

PP. Eight connected 0,2 ml tubes with attached domed cap strips.

Supplier: Brand

PP. Eight connected 0,2 ml tubes and separate domed or flat caps in strips of 8.

Supplier: Brand

PP. Eight connected 0,2 or 0,15 ml PCR tubes with attached, flat individual caps.