568 Results for: "AMAS+(N-Succinimidyl+maleimidoacetate)"

Supplier: AAT BIOQUEST

Advances in spectral flow cytometers have expanded applications and capabilities beyond conventional flow cytometry.

Supplier: AAT BIOQUEST

Advances in spectral flow cytometers have expanded applications and capabilities beyond conventional flow cytometry.

Supplier: AAT BIOQUEST

The succinimidyl esters of 5-TAMRA, 6-TAMRA or the mixed isomers are the primary labeling reagents for the preparation of orange fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry.

Supplier: Merck Millipore (Calbiochem‎)

Heterobifunctional reagent useful for enzyme labeling of antibodies and antibody fragments. Cross-links both a sulfhydryl group and a free amine group. Useful for sequential conjugation to minimize polymerization.
Reacts with primary amines and sulfhydryl groups.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications.

Supplier: AAT BIOQUEST

mFluor™ Green 620 dye is a unique dye that is well excited by the green laser at 532 nm to give red fluorescence.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Supplier: Biotium

5-FAM SE is an amine-reactive form of 5-carboxyfluorescein single isomer.

Supplier: AAT BIOQUEST

Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Supplier: Biotium

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

Supplier: TCI

Succinimidyl-6-[[7-(N,N-dimethylaminosulphonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoate ≥98.0% (by HPLC, total nitrogen)

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce Premium Grade DSP is our highest quality formulation of this popular amine-reactive, cleavable crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: AAT BIOQUEST

iFluor® Ultra 594 is an exceptionally bright red fluorescent dye excitable by 561 nm or 594 nm laser lines, with a peak emission at 601 nm.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications.

Supplier: AAT BIOQUEST

mFluor™ Blue 590 dye can be well excited with blue laser at 488 nm.

Supplier: AAT BIOQUEST

mFluor™ Red 780 dyes are an excellent alternative to APC-Alexa Fluor® 750 tandems since they have the spectral properties equivalent to those of APC-Alexa Fluor® 750 conjugates.

Supplier: AAT BIOQUEST

Advances in spectral flow cytometers have expanded applications and capabilities beyond conventional flow cytometry.

Supplier: AAT BIOQUEST

TF1 is designed to be a superior fluorophore alternative to EDANS.

Supplier: AAT BIOQUEST

mFluor™ Blue 630 dye can be well excited with blue laser at 488 nm.

Supplier: TCI

Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt)

Supplier: AAT BIOQUEST

mFluor™ Blue 570 dyes are an excellent alternative to RPE since they have the spectral properties equivalent to those of RPE conjugates.

Supplier: AAT BIOQUEST

TF4 is designed to be a superior fluorophore alternative to ROX and Texas Red®.

Supplier: AAT BIOQUEST

iFluor® Ultra 750 is a bright NIR fluorescent dye excitable by 633 nm to 685 nm laser lines, with a peak emission at 773 nm.

Supplier: AAT BIOQUEST

iFluor® Ultra 647 is an exceptionally bright far-red fluorescent dye excitable by 594 nm or 633 nm laser lines, with a peak emission at 670 nm.

Supplier: Biotium

6-SFX (full name: 6-(Fluorescein-6-carboxamido)hexanoic acid, succinimidyl ester, single isomer) has a 6-atom aminohexanoyl spacer group that separates the fluorescein dye and the amine-reactive group.

Supplier: AAT BIOQUEST

TQ1 is designed to be a superior quencher alternative to DABCYL.

Supplier: AAT BIOQUEST

The amine-reactive 7-diethylaminocoumarin-3-carboxylic acid, succinimidyl ester can be used to create blue-fluorescent bioconjugates.