568 Results for: "AMAS+(N-Succinimidyl+maleimidoacetate)"

Supplier: AAT BIOQUEST

iFluor® 780 dyes have fluorescence excitation and emission maxima of ~784 nm and ~808 nm respectively.

Supplier: G-Biosciences

(Bis(2-[Succinimidooxycarbonyloxy]ethyl) sulfone Homobifunctional Cross Linkers.

Supplier: TCI

7-Hydroxy-4-methylcoumarin-3-acetic acid succinimidyl ester ≥90.0% (by HPLC, total nitrogen)

Supplier: Biotium

6-ROX, SE (6-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxy-X-rhodamine single isomer.

Supplier: AAT BIOQUEST

5-(and-6)-Carboxy SNARF-5F Succinimidyl Ester is an excellent amine-reactive building block that can be readily used for developing SNARF-5F pH probes.

Supplier: AAT BIOQUEST

SMCC is a non-cleavable and membrane permeable crosslinker with a spacer arm of 8.3 Å. It contains an amine-reactive succinimidyl ester (SE) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7 to 9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5 to 7.5 to form stable thioether bonds. The SE group of SMCC is reacted with lysine residues on the carrier protein, converting them to reactive maleimides. SMCC is a reagent that is widely used for generating stable maleimide-activated carrier proteins that can spontaneously react with sulfhydryls. Alternatively these relatively stable maleimide-activated intermediates may be lyophilized and stored for later conjugation to a hapten.

Supplier: AAT BIOQUEST

The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of Invitrogen).

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Supplier: AAT BIOQUEST

Succinimidyl 4,4-azipentanoate, also known as NHS-Diazirine, represents a significant breakthrough in crosslinking technology.

Supplier: AAT BIOQUEST

5-(and-6)-Carboxy SNARF-4F Succinimidyl Ester is an excellent amine-reactive building block that can be readily used for developing SNARF-4F pH probes.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

Supplier: AAT BIOQUEST

ReadiLeave™ Reversible (RLR) Biotin is a newly developed biotin derivative that has significantly reduced affinity to avidin (including streptavidin) to make the binding of RLR biotin and streptavidin readily reversible when needed.

Supplier: AAT BIOQUEST

An amine-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Supplier: AAT BIOQUEST

The amine-reactive Texas Red®-X, succinimidyl ester is a superior replacement to Texas Red (Texas Red® is the trademark of Invitrogen).

Supplier: Thermo Fisher Scientific

SATP (and SATA) are reagents for introducing protected sulfhydryls into proteins, peptides and other molecules. for covalent modification of primary amines and addition of a protected sulphhydryl group.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SIAB is a mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.

Supplier: Biotium

6-FAM, SE (full name: 6-Carboxyfluorescein, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxyfluorescein single isomer.
6-FAM SE is an amine-reactive green fluorescent dye widely used for labeling oligonucleotides or other biomolecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5.

Supplier: AAT BIOQUEST

6-FAM, SE is the amine-reactive succinimidyl ester of single isomer 6-FAM acid.

Supplier: AAT BIOQUEST

5-FAM, SE is the amine-reactive succinimidyl ester of single isomer 5-FAM acid.

Supplier: Biotium

5-ROX, SE (5-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 5-carboxy-X-rhodamine single isomer.

Supplier: Merck Millipore (Calbiochem‎)

A useful agent for introducing thiol groups, in protected form, into various proteins. Reacts with primary amines to add protected sulfhydryl groups. When treated with hydroxylamine, yields a free sulfhydryl group that can be conjugated to maleimide-modified proteins.

Supplier: TCI

LC-SMCC (N-Succinimidyl 6-[[4-(N-Maleimidomethyl)cyclohexyl]carboxamido]hexanoate) ≥98.0% (by HPLC)

Supplier: AAT BIOQUEST

DABCYL, SE is the amino-reactive form of DABCYL, and widely used to prepare a variety of FRET-based probes that contain DABCYL.

Supplier: Apollo Scientific

alpha-(9-Fluorenylmethyloxycarbonyl)amino-omega-carboxy succinimidyl ester PEG

Supplier: Biotium

5-ROX-PEO8 SE (full name: 5-Carboxy-X-rhodamine-PEO8-propionate, succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates the red fluorescent ROX dye and the amine-reactive SE group.

Supplier: Biotium

6-TAMRA SE (full name: 6-Carboxytetramethylrhodamine succinimidyl ester) is the amine-reactive form of Amine-reactive form of Carboxytetramethylrhodamine (TAMRA) single isomer. Supplied at 90 mg/mL (0.17 M) in anhydrous DMSO. The solution is suitable for labeling oligonucleotides.

Supplier: AAT BIOQUEST

mFluor™ Blue 620 dye can be well excited with blue laser at 488 nm.