Dye terminator removal, illustra™ AutoSeq™ G-50 columns

Supplier: Cytiva

illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

Expand 3 Items

Sodium tetrachloroaurate dihydrate ≥99.99% (metals basis) Au 49-50%, Premion®

Supplier: Thermo Fisher Scientific

Sodium tetrachloroaurate dihydrate ≥99.99% (metals basis) Au 49-50%, Premion®

Expand 2 Items

illustra™ ProbeQuant™ G-50 micro columns

Supplier: Cytiva

ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.

Expand 1 Items

Nickel, gauze woven from wire 50 mesh, Ø 0.05 mm (0.002 in)

Supplier: Thermo Fisher Scientific

Nickel, gauze woven from wire 50 mesh, Ø 0.05 mm (0.002 in)

Expand 3 Items

Platinum 10% on activated carbon wetted with ca. 50% water, powder, unreduced

Supplier: Thermo Fisher Scientific

Platinum 10% on activated carbon wetted with ca. 50% water, powder, unreduced

Expand 3 Items

Copper(II) oxide, granular for elementary analysis pH 7 (50 g/l, H₂O, 20 °C) (slurry), Supelco®

Supplier: Merck

Copper(II) oxide, granular for elementary analysis pH 7 (50 g/l, H₂O, 20 °C) (slurry), Supelco®

Expand 2 Items

Propidium iodide 50 ug/mL in PBS

Supplier: Biotium

Propidium Iodide is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.

Expand 1 Items

Tantalum, gauze woven from wire 50 mesh, Ø 0.076 mm (0.003 in)

Supplier: Thermo Fisher Scientific

Tantalum, gauze woven from wire 50 mesh, Ø 0.076 mm (0.003 in)

Expand 1 Items

Gel filtration media, Sephadex™ G-50

Supplier: Cytiva

Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

Expand 5 Items

Platinum 10% on activated carbon wetted with ca. 50% water, powder, reduced

Supplier: Thermo Fisher Scientific

Platinum 10% on activated carbon wetted with ca. 50% water, powder, reduced

Expand 3 Items

Anti-CD44 Mouse Monoclonal Antibody [clone: OX-50] PE (Phycoerythrin)

Supplier: Abcam

PE Mouse monoclonal [OX-50] to CD44.

Expand 1 Items

Reactive black 5 ≥50 % (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Reactive Black 5 (RB5) has been: used in an optimization study on the photocatalytic degradation of RB5 dye in synthetic greywater effluent (i.e. aqueous RB5 in distilled water), in order to understand the effects of different photoreactor parameters on the degradation kinetics. As a textile dye to investigate the anaerobic reduction in upflow stirred packed-bed reactors (USPBRs) with biological activated carbon (BAC) system. Used in the performance evaluation of dye removal forlarge molecule substance.

Expand 1 Items

DNA ladder 50 to 1500 bp, AMPIGENE®

Supplier: Enzo Life Sciences

AMPIGENE® DNA ladder contains DNA fragments ranging from 50 bp to 1500 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 200 bp, 500 bp and 1500 bpto serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.

Expand 1 Items

Silver, gauze woven from wire 50 mesh, Ø 0.0764 mm (0.003 in)

Supplier: Thermo Fisher Scientific

Silver, gauze woven from wire 50 mesh, Ø 0.0764 mm (0.003 in)

Expand 3 Items

Sodium hydroxide 50% in aqueous solution Reag. Ph. Eur. 1081406 carbonate-free

Supplier: VWR Chemicals

Sodium hydroxide 50% in aqueous solution Reag. Ph. Eur. 1081406 carbonate-free

Expand 1 Items

Anti-50S ribosomal protein L7/L12 Rabbit Polyclonal Antibody

Supplier: Abcam

Rabbit polyclonal to 50S ribosomal protein L7/L12.

Expand 1 Items

Sodium hydroxide 0.001 mol/l (0.001 N) in methanol 50% Reag. Ph. Eur. 1081403

Supplier: VWR Chemicals

Sodium hydroxide 0.001 mol/l (0.001 N) in methanol 50% Reag. Ph. Eur. 1081403

Expand 1 Items

Hydroxylamine solution 50% (w/w) in water for TMT experiments

Supplier: Thermo Fisher Scientific

Thermo Scientific 50% Hydroxylamine is a 10X quenching reagent for amine-reactive isobaric mass tag labeling experiments.

Expand 1 Items

Polyethyleneimine, M.W. ∼ 60,000 50% (w/w) in aqueous solution

Supplier: Thermo Fisher Scientific

CAS No.: 9002-98-6

Expand 3 Items

2-Pyridineboronic acid-N-phenyldiethanolamine ester 50 - 70%

Supplier: Thermo Fisher Scientific

see CofA for exact composition

Expand 3 Items

Copper, gauze woven from wire 50 mesh, Ø 0.23 mm (0.009 in)

Supplier: Thermo Fisher Scientific

Copper, gauze woven from wire 50 mesh, Ø 0.23 mm (0.009 in)

Expand 2 Items

Anti-Hepatitis C Virus Core 1b Mouse Monoclonal Antibody [clone: C7-50]

Supplier: Abcam

Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b.

Expand 1 Items

Palladium 5% on activated carbon wetted with ca. 50% water, powder Type A405023-5

Supplier: Thermo Fisher Scientific

Palladium 5% on activated carbon wetted with ca. 50% water, powder Type A405023-5

Expand 3 Items

Perfluorokerosene 50% in PFK low boiling, super-high boiling for mass spectrometry

Supplier: TCI

Perfluorokerosene 50% in PFK low boiling, super-high boiling for mass spectrometry

Expand 1 Items

Lutetium(III) oxide ≥99.995% (metals basis), REacton® (REM ≤ 50 ppm)

Supplier: Thermo Fisher Scientific

Lutetium(III) oxide ≥99.995% (metals basis), REacton® (REM ≤ 50 ppm)

Expand 2 Items

Anti-EIF5A Rabbit Recombinant Antibody [clone: 50]

Supplier: Abnova

Anti-EIF5A Rabbit Recombinant Antibody [clone: 50]

Expand 1 Items

Freezer Storage Boxes for 50 ml Centrifuge Tubes

Supplier: Labcon

Labcon 16 or 36 Place Freezer Storage Boxes for 50 ml centrifuge tubes. These boxes are designed to hold 50 ml tubes and are perfect for long term storage of frozen samples.