Specifications
- Antigen name:SARS-CoV-2 nsp7
- Clonality:Monoclonal
- Clone:HL1301
- Conjugation:Unconjugated
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Virus
- Western blot:Yes
- Format:Azide and BSA free
- Gene ID:ORF1ab
- Antigen synonyms:nsp7 replicase|Non-structural protein 7
- Purity:Protein A purified.
- Storage buffer:PBS
- Storage temperature:Store at 4 °C short term. Aliquot and store at −20 °C long term. Avoid freeze-thaw cycles.
- Immunogen:Full length SARS-CoV-2 nsp7 recombinant protein.
- Pk:100 µl
Specifications
About this item
SARS-CoV-2 Nonstructural Protein 7 (NSP7) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP7 is 83 amino acids (aa) with a theoretical molecular weight of 9,2 kDa (1,2).
- Immunocytochemistry/Immunofluorescence: Assay dependent
- Western blot: 1:1000 to 1:10000
The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP7 has 98,8% sequence identity and 100% sequence similarity (1). Functionally, SARS-Cov-2 NSP7 forms a complex with NSP8 and NSP12 to generate NSP8's RNA polymerase activity (1-3). In SARS-CoV, NSP7 and NSP8 are required for viral survival and form a channel-like, cylinder supercomplex (3). The NSP7-NSP8-NSP12 complex is considered the minimal core complex for RNA synthesis (4). While structurally similar to SARS-CoV, the components of the NSP7-NSP8-NSP12 complex of SARS-CoV-2 have reduced polymerase activity and thermostability (4).
Type: Primary
Antigen: SARS-CoV-2 nsp7
Clonality: Monoclonal
Clone: HL1301
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Virus