A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

• Stain yeast bud scars as well as the cell membrane of gram-positive bacteria and mammalian cells or tissues
• Suitable for Western Blot, ELISA, immunohistochemistry and other standard immunoassay applications
• A retrograde or anterograde neuronal tracer
• Withstands fixation and permeabilisation

WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections or for western blotting. WGA can be used as a gram stain to fluorescently label gram-positive bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae.

Find the Right Stain for Your Application.
WGA and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins. The presence and distribution of these targets vary between cell types and tissues. As a result, other cell surface stains or other lectin conjugates, Concanavalin A (Con A) conjugates and PNA lectin conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells and withstand fixation and permeabilisation. When cells are fixed and permeabilised before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type.