Specifications

• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Lyopilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.; Measured by its binding ability in a functional ELISA. Immobilised Human PD-L1, Fc Tag at 5 μg/ml (100 µl/well) can bind Biotinylated Human PD-1, Fc,Avitag with a linear range of 0,02-0,32 μg/ml (QC tested).; Measured by its binding ability in a functional ELISA. Immobilised Nivolumab at 2 μg/ml (100 µl/well) can bind Biotinylated Human PD-1, Fc,Avitag with a linear range of 0,2-6 ng/ml (Routinely tested).; Measured by its binding ability in a functional ELISA. Immobilised Biotinylated Human PD-1, Fc,Avitag at 1 μg/ml (100 µl/well) on streptavidin precoated (0,2 μg/well) plate, can bind Mouse PD-L2, His Tag with a linear range of 20-156 ng/ml (Routinely tested).; Serial dilutions of nivolumab were added into Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified): Biotinylated Human PD-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0,3789 μg/ml (Routinely tested).; Serial dilutions of nivolumab were added into Human PD-L2, Mouse IgG1 Fc Tag: Biotinylated Human PD-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0,2823 μg/ml (Routinely tested); Loaded Biotinylated Human PD-1, Fc,Avitag on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) with an affinity constant of 2,5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Loaded Biotinylated Human PD-1, Fc,Avitag on SA Biosensor, can bind Human PD-L2, His Tag (SPR verified) with an affinity constant of 0,31 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  Q15116-1
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  CD279|PDCD1|PD1|SLEB2
• Protein/peptide name:
  PD-1
• Purity:
  >95%
• Molecular weight:
  44,4 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyopilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT