Specifications

• Pack type:
  1 mg
• Pk:
  1 mg
• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation.; Measured by its binding ability in a functional ELISA. Immobilised Human 4‑1BB Ligand, Fc Tag at 1 μg/ml (100 μl/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0,6-5 ng/ml (QC tested).; Measured by its binding ability in a functional ELISA. Immobilised Human 4‑1BB Ligand, His Tag at 2 μg/ml (100 μl/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0,1-4 ng/ml (Routinely tested).; Flow Cytometry assay shows that Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0,03 μg/ml (Routinely tested)
• Gene ID:
  Q07011-1
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  CD137|TNFRSF9|ILA|CDw137|4-1BB
• Protein/peptide name:
  4-1BB
• Purity:
  >95%
• Molecular weight:
  44,2 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT