Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 0,1 EU per μg by the LAL method.
• Biological activity:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4, Normally trehalose is added as protectant before Lyophilisation,; Measured by its binding ability in a functional ELISA, Immobilised Human OX40, Fc Tag (Cat, No, OX0-H5255) at 0,5 μg/ml (100 μl/well) can bind Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat, No, OXL-H5250) with a linear range of 0,8-13 ng/ml (QC tested),; Loaded Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat, No, OXL-H5250) on Protein A Biosensor, can bind Human OX40, His Tag (Cat, No, OX0-H5224) with an affinity constant of 2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested),; FACS assay shows that recombinant Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat, No, OXL-H5250) can bind to 293T cell overexpressing human OX40, The concentration of OX40 Ligand is 0,003 μg/ml (Routinely tested),; FACS analysis shows that the binding of Human OX40 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat, No, OXL-H5250) to 293T overexpressing OX40 was inhibited by increasing concentration of neutralizing anti-OX40 antibody, The concentration of OX40 Ligand used is 0,002 μg/ml, IC50=3,021 μg/ml (Routinely tested).
• Gene ID:
  P23510-1
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  TNFSF4|CD134L|TXGP1|OX40L|Glycoprotein Gp34|CD134 ligand|CD252
• Protein/peptide name:
  OX40 Ligand
• Purity:
  >95%
• Molecular weight:
  42,3 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4, Normally trehalose is added as protectant before Lyophilisation.
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT