Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit
Catalog # ENZOBMLAK5550001
Supplier: ENZO LIFE SCIENCES
CAS Number:
Specifications
- Pk:1 KIT
- Description:Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit
- Seq:02
- Storage conditions:–80 °C (long term)
- Application:Fluorescent detection, HTS activity assay, cell-based assays
- Size:96 well
Specifications
About this item
A Fluor-de-Lys® fluorescent assay system. The SIRT1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT1 included in the kit.
- Useful for inhibitor screening or characterizing enzyme kinetics
- Includes optimal substrate selected from a panel of acetylated sites in p53 and histones
The Fluor-de-Lys® SIRT1 assay is based on the Fluor de Lys® SIRT1 Substrate and Fluor de Lys® Developer II combination. The assay procedure has two steps. First, the Fluor de Lys® SIRT1 Substrate, which contains a peptide comprising amino acids 379-382 of human p53 (Arg-His-Lys-Lys(Ac)). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer II produces a fluorophore.
Delivery: Kit includes SIRT1 (Sirtuin 1, hSir2SIRT1) (human, recombinant), FLUOR DE LYS® SIRT1 Deacetylase Substrate, 5X FLUOR DE LYS® Developer II Concentrate, NAD (Sirtuin Substrate), Nicotinamide (Sirtuin Inhibitor), Resveratrol (Sirtuin Activator), Suramin sodium (Sirtuin Inhibitor), FLUOR DE LYS® Deacetylated Standard, Sirtuin Assay Buffer, Half volume microplates.