FLUOR DE LYS® HDAC1 fluorometric drug discovery assay kit
Catalog # ENZOBMLAK5110001
Supplier: ENZO LIFE SCIENCES
CAS Number:
Specifications
- Pk:1 KIT
- Description:FLUOR DE LYS® HDAC1 fluorometric drug discovery assay kit
- Seq:02
- Storage conditions:–80 °C (long term)
- Application:Fluorescent detection, HTS activity assay, cell-based assays
- Size:96 well
Specifications
About this item
The HDAC1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC1 included in the kit.
- Useful for inhibitor screening or characterizing enzyme kinetics
- Includes optimal substrate selected from a panel of acetylated sites in p53 and histones
The Fluor de Lys® HDAC1 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC1. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
Delivery: Kit includes HDAC1 (Histone Deacetylase 1) (human, recombinant), FLUOR DE LYS® SIRT1 (Deacetylase Substrate), 5X FLUOR DE LYS® Developer II Concentrate, Trichostatin A (HDAC Inhibitor), FLUOR DE LYS® Deacetylated Standard, HDAC Assay Buffer II, HDAC Assay Buffer, Half Volume Microplates.