Minimum Essential Medium (MEM) is one of the earliest modifications of Basal Medium Eagle (BME) containing amino acid concentrations that more closely mimic those found in mammalian cells. MEM has been used with serum supplementation on a broad range of cell types including suspension and adherent mammalian cells, HeLa, BHK-21, HEK-293, HEp-2, HT-1080, MCF-7,fibroblasts and primary rat astrocytes.

• Available with or without L-glutamine, sodium pyruvate, or phenol red
• Multiple modifications and reduced serum options
• Hydrated using WFI quality water
• 0,1 μm sterile filtered
• Manufactured using ISO 9001:2000-certified processes

Numerous other modifications of MEM have also been developed. MEM contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin and ascorbic acid. MEM has been used for many cell types grown as monolayers.

MEM is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. Suspension modifications replace calcium with magnesium ions to inhibit cell attachment.

ADCF=Animal derived component free