- Antibody type:Secondary
- Antigen name:Immunoglobulin G
- Antigen symbol:IgG
- Clonality:Polyclonal
- Conjugation:FITC (Fluorescein Isothiocyanate)
- ELISA:Yes
- Flow cytometry:Yes
- Host:Donkey
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Isotype:IgG
- Reactivity:Sheep
- Western blot:Yes
- Environmentally Preferable:
- Cross adsorption:Yes
- Format:Purified IgG
- Form:Clear, fluorescent yellow liquid
- Storage buffer:10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1% (w/v) BSA, Protease/IgG free
- Storage temperature:2...8 °C
- Concentration:1,5 mg/ml (E 1% at 280 nm = 13,0)
- Shipping temperature:2...8 °C
- Immunogen:Sheep IgG
- Tested applications:Fluoresence ELISA, Fluorescence-Activated Cell Sorting
- Purification:Affinity purified using solid phaseSheep IgG
- Size:2,0 mg
- Pk:2 mg
ImmunoReagents’ FITC Conjugates are used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. Fluorescein is typically excited by the 488 nm line of an argon laser, and emission is collected at 518 nm. FITC antibody conjugates are yellow-orange in Colour but emit a yellow-green Colour upon Em 518 nm. FITC antibody conjugates are sensitive to light. The typical FITC to antibody ratio (F/P) is >4.0 Excitation/Emission = 494 nm/518 nm Emission Colour = Green (Similar Dyes: Alexa Fluor 488, Cy2, DyLight ® 488).
- Origin: US
ImmunoReagents' antibodies are suitable for most immunoassay formats and purified, cross adsorbed and conjugated based on the final product specfications.
Products will be accompanied with a standard product CoA to include application, purity and cross reactivity profiles.
Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: FITC (Fluorescein Isothiocyanate)
Epitope:
Host: Donkey
Isotype: IgG
Reactivity: Sheep