Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Western blotting detection reagent, Amersham™ ECL™ start
Supplier: Cytiva
Chemiluminescence detection reagent for detection of high to medium abundant proteins.
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Perfect DNA™ ladders
Supplier: Merck Millipore (Novagen)
The Perfect DNA™ ladders contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities and cover a wide range of DNA sizes.
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DNA ladder, 100 bp
Supplier: PanReac AppliChem
This DNA ladder is ideal for determining the size of double-stranded DNA ranging from 100 to 1,000 base pairs. The ladder consists of ten fragments that range in size from 100 to 1,000 bp in 100 bp increments. The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are blunt-ended.
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DNA QuantLadders
Supplier: LONZA
QuantLadders are prepared from five highly purified DNA fragments that provide visual quantitation of DNA within ±1% accuracy. The DNA QuantLadder provides each DNA fragment in perfect relative mass equivalence at 100 ng, 70 ng, 50 ng, 20 ng and 10 ng. The reverse QuantLadder simply reverses the mass equivalence order of the QuantLadder.
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Substrate for Western blotting, ECF™
Supplier: Cytiva
ECF™ substrate for Western blotting
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Amersham ECL Direct Labeling System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
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Protein markers for gel electrophoresis
Supplier: PanReac AppliChem
The main applications for prestained protein markers are the monitoring of protein migration during SDS-PAGE, verification of Western transfer efficiency on membranes, and estimation of protein sizes on SDS-polyacrylamide gels and Western blots.
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DNA ladder
Supplier: LONZA
Thse DNA ladders provides rapid and easy size determination of the DNA samples. Each band has an ideal 50 % G-C content for precise sizing and sharp resolution. It is free from contaminating plasmid DNA with minimal background.
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CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva
Supplier: Cytiva
CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.
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Western blotting system, Amersham ECL™
Supplier: Cytiva
Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.
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Amersham™ ECL Direct™ Detection Reagents, Cytiva
Supplier: Cytiva
Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.
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Antibody diluent, MP Biomedicals
Supplier: MP Biomedicals
This antibody diluent is compatible with common stabilising compounds at pH 7,3.
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Western blotting combination pack, Amersham ECL Plex™
Supplier: Cytiva
Amersham ECL Plex™ Western blotting combination packs offer a complete system for fluorescent Western blotting, with improved formulation Cy3 conjugates for Lower background.
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Prestained protein markers, Peacock™
Supplier: Biotium
A visible three-colour protein marker for SDS-PAGE or western blotting, with 10 bands ranging from 10 to 180 kDa.
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Protein markers, ProSieve™ Colour
Supplier: LONZA
ProSieve™ Color protein marker have been labelled with three different fluorescent dyes. These markers are supplied as a lyophilised solid containing the buffer salts and detergent found in the typical Laemmli buffer system. It contains nine proteins with approximate masses of 10, 15, 20, 25, 40, 50, 80, 125, and 190 kDa.
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Western blotting markers, Perfect Protein™, Novagen®
Supplier: Merck Millipore (Novagen)
The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.
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Western blotting blocking reagent, Amersham ECL™ Prime
Supplier: Cytiva
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
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Amersham ECL Direct Labeling and Detection System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
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pI markers
Supplier: Cytiva
pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.
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Protein and peptide molecular weight markers
Supplier: Cytiva
For determination of molecular weight of uncharacterised protein samples with precision by comparison with carefully selected standards.
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Molecular weight markers, Rainbow™
Supplier: Cytiva
Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.
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Protein standards
Supplier: Genscript
GenScript offers a wide selection of Protein molecular weight standards for SDS-PAGE and Western blot, including prestained, unstained, peptide, Western-viewable, and other standards.
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Molecular weight markers, Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers
Supplier: Cytiva
Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers are protein molecular weight markers optimised for use with the ECL™ Plex™ Western blotting detection system. They are designed to provide visible marker bands on gels and membranes, as well as fluorescent images using Cy3 and Cy5 channels.
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Western blotting markers, Amersham™ ECL™ DualVue™
Supplier: Cytiva
Amersham™ ECL™ DualVue™ Western blotting markers perform two key functions. Firstly, the prestained marker proteins confirm protein transfer in addition to clearly defining blot orientation. Secondly, the recombinant tagged proteins are detected on film or by CCD imaging in conjunction with the target protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target protein(s) and markers.
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Diluent, green
Supplier: IMMUNOLOGIC
Bright Diluent is provided as a ready to use normal antibody diluent intended for diluting and stabilising antibodies. It eliminates background encountered in immunohistochemistry staining procedures. The diluent contains complex proteins and non-protein mixtures which eliminates immunohistostaining background by blocking Fc receptors and prevents non-specific protein-protein interactions.
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Antibody diluent clear
Supplier: IMMUNOLOGIC
Universal IHC Blocking/Diluent is specially formulated to stabilise antibodies and eliminate backgrounds encountered in immunohistochemistry staining procedures. The diluent contains complex proteins and non-protein mixtures which eliminate histostaining background by blocking Fc receptors and prevents non-specific protein-protein interactions.
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HRP chemiluminescent substrate, UptiLight™
Supplier: Uptima
UptiLight™ provides HRP chemiluminescent substrate formulations optimised for Western-Blotting (and ELISA) applications. UptiLight™HS HRP is highly sensitive with low background and cost-effective, designed for Western Blotting standard applications.
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Substrate for the detection of immobilised HRP
Supplier: Uptima
Substrate optimised for the detection of immobilised HRP in Western, Southern, Northern and dot blot applications.
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IEF carrier ampholytes, SERVALYT™
Supplier: Serva
IEF carrier ampholyte, SERVALYT™, pH 6 - 7, analytical grade
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DNA Ladder 1 kb
Supplier: STEMCELL Technologies
DNA ladder consisting of 13 fragments from 250 to 10000 base pairs for determining the size of double-stranded DNA by gel electrophoresis.