You searched for: Enzyme Assays

Supplier: AAT BIOQUEST

Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.

Supplier: AAT BIOQUEST

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

Supplier: AAT BIOQUEST

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

Supplier: AAT BIOQUEST

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.

Supplier: AAT BIOQUEST

The traditional Bradford protein assay is widely used for quantifying protein concentrations.

Supplier: AAT BIOQUEST

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

Supplier: AAT BIOQUEST

E. coli beta-galactosidase is a 464 Kd tetramer.

Supplier: AAT BIOQUEST

Monitoring of various protease activities has become a routine task for many biological laboratories.

Supplier: AAT BIOQUEST

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized liver and present in blood.

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® fluorimetric neutrophil elastase activity assay kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity.

Supplier: AAT BIOQUEST

Hydrogen peroxide (H₂O₂) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

Supplier: AAT BIOQUEST

Ammonia is an important source of nitrogen for living systems.

Supplier: AAT BIOQUEST

Urea is the final degradation product of protein and amino acid metabolism in animals.

Supplier: AAT BIOQUEST

Zinc is an essential trace mineral element that plays an important role in a number of biological processes.

Supplier: AAT BIOQUEST

Alpha-ketoglutarate (α-Ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.

Supplier: AAT BIOQUEST

Zinc is an essential trace mineral element that plays an important role in a number of biological processes.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.

Supplier: AAT BIOQUEST

The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.

Supplier: AAT BIOQUEST

The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.

Supplier: AAT BIOQUEST

Coenzyme A (CoA) is a universal and essential cofactor in all forms of cellular life acting as a principal acyl carrier in numerous biosynthetic, energy-yielding, and degradative pathways.

Supplier: Thermo Fisher Scientific

The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Supplier: G-Biosciences

SAM510™ assay is a continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.

Supplier: G-Biosciences

The Fluorescent β-Galactosidase assay is used for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The Fluorescent β-Galactosidase assay (MUG) provides a 96-well assay format for galactosidase activity that is suitable for high throughput applications. The production of the fluorphore is monitored at an emission/excitation wavelength of 365/460 nm.

Supplier: Thermo Fisher Scientific

The Pierce™ Kinase Enrichment Kits utilise an ActivX™ ATP or ADP probe to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.

Supplier: G-Biosciences

The Protease Screening Kit provides a simple and quick method for testing samples for proteolysis. Simply incubate sample in the reagent provided and obtain results.

Supplier: Thermo Fisher Scientific

The Active GTPase Pull-Down and Detection Kits allows the monitoring of changes in GTPase activity levels by enriching and detecting the active (GTP-bound) state of a particular GTPase.

Supplier: G-Biosciences

β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.