You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Amplite® Fluorimetric DPP4 Inhibitor Screening Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors.

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Pierce™ Alkaline Phosphatase, Biotinylated

Supplier: Thermo Fisher Scientific

Biotinylated alkaline phosphatase is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.

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Pierce™ Firefly Luc One-Step Glow Assay Kit

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.

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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit

Supplier: AAT BIOQUEST

Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.

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Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Supplier: AAT BIOQUEST

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Amplite® Renilla Luciferase Reporter Gene Assay Kit Bright Glow

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Gaussia Luciferase Reporter Gene Assay Kit Bright Glow

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Fluorimetric Lipase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity.

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Amplite® Cholesterol Quantitation Kit

Supplier: AAT BIOQUEST

Cholesterol is required to build and maintain cell membranes.

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Amplite® Colorimetric NADPH Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Acetylcholine Assay Kit Red Fluorescence

Supplier: AAT BIOQUEST

Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways.

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MMP Green™ Substrate

Supplier: AAT BIOQUEST

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Rapid Colorimetric Total Protein Thiol Quantitation Assay Kit

Supplier: AAT BIOQUEST

Protein thiols are very important to protein structure, protein function and biological system redox environment.

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Amplite® Colorimetric Hexokinase Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Hexokinase Assay Kit is designed to accurately measure the activity of hexokinases from various sources using a simple and quick protocol.

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Amplite® Colorimetric Hydrogen Peroxide Assay Kit

Supplier: AAT BIOQUEST

Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

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Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit Blue Fluorescence

Supplier: AAT BIOQUEST

Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative.

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Amplite® Fluorimetric Total NADP and NADPH Assay Kit Red Fluorescence

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit Red Fluorescence

Supplier: AAT BIOQUEST

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections.

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Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Screen Quest™ fluorimetric neutrophil elastase inhibitor screening kit offers a fast, sensitive, and high-throughput method for screening and characterising potential inhibitors of NE.

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Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.

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Amplite® Colorimetric Sphingomyelinase Assay Kit Blue Color

Supplier: AAT BIOQUEST

Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.

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Amplite® Luminometric Alkaline Phosphatase Assay Kit Luminescence

Supplier: AAT BIOQUEST

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

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Amplite® Colorimetric Acetylcholinesterase Assay Kit

Supplier: AAT BIOQUEST

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

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Amplite® Fluorimetric Acetylcholinesterase Assay Kit Red Fluorescence

Supplier: AAT BIOQUEST

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

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Amplite® Fluorimetric NAD Assay Kit Blue Fluorescence

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.

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Amplite® Colorimetric Bradford Protein Quantitation Assay Kit

Supplier: AAT BIOQUEST

The traditional Bradford protein assay is widely used for quantifying protein concentrations.

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Amplite® Fluorimetric L-Alanine Assay Kit

Supplier: AAT BIOQUEST

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

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Amplite® Fluorimetric Beta-Galactosidase Assay Kit Green Fluorescence

Supplier: AAT BIOQUEST

E. coli beta-galactosidase is a 464 Kd tetramer.

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Amplite® Universal Fluorimetric Protease Activity Assay Kit Green Fluorescence

Supplier: AAT BIOQUEST

Monitoring of various protease activities has become a routine task for many biological laboratories.

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Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit

Supplier: AAT BIOQUEST

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized liver and present in blood.