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You searched for: Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

VWR® Antibody signal enhancer

VWR® Antibody signal enhancer

Supplier: VWR Chemicals

Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.

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ZnAF-2.tetrahydrochloride

Supplier: ENZO LIFE SCIENCES

Fluorescent probe for Zn2+

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SeramunBlock

Supplier: Apollo Scientific

Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.

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Antibody labelling kits, Mix-n-Stain™ CF™ Dye

Antibody labelling kits, Mix-n-Stain™ CF™ Dye

Supplier: Biotium

Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.

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femtoCHROMO™- AP Substrate for chromogenic detection

femtoCHROMO™- AP Substrate for chromogenic detection

Supplier: G-Biosciences

A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.

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CF™ dye bovine serum albumin conjugates

Supplier: Biotium

Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.

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Green 496 dUTP (lyophilized)

Green 496 dUTP (lyophilized)

Supplier: ENZO LIFE SCIENCES

Green 496 dUTP (lyophilised)

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Aqua 431 dUTP (lyophilised)

Supplier: ENZO LIFE SCIENCES

Aqua 431 dUTP (lyophilised)

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ZnAF-2 DA

Supplier: ENZO LIFE SCIENCES

Fluorescent probe for Zn²⁺. Cell permeable.

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Reactive quencher dyes

Supplier: Biotium

Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

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Biotin kits, Micro HOOK™

Supplier: G-Biosciences

The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

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Organelle-ID™ RGB reagent I

Supplier: ENZO LIFE SCIENCES

Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids

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Maxi fluorescent antibody labelling kits, Mix-n-Stain™, 1 mg labelling

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labelling, for conjugation of 1 mg IgG with fluorescent CF® dyes or other dyes.

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B-Phycoerythrin (BPE)

B-Phycoerythrin (BPE)

Supplier: Columbia Biosciences

Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.

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Dye conjugates dUTP CF®

Supplier: Biotium

CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

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Biotin SE protein labelling kit

Supplier: Biotium

This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.

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HRP-conjugate stabiliser, SeramunStab® STNP

Supplier: Apollo Scientific

Liquid product red odourless pH 6,7 to 6,9. Protein free Stabiliser. Enzyme activity preservation by special chemistry.

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Bilirubin conjugate, sodium salt

Supplier: Cayman Chemical

Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.

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Calmodulin Resin Kit for CBP purification

Calmodulin Resin Kit for CBP purification

Supplier: G-Biosciences

The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.

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ExoBrite™ CTB EV Staining Kits, Biotium

Supplier: Biotium

ExoBrite™ CTB EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ CTB EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.

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Green 496 dUTP

Supplier: ENZO LIFE SCIENCES

Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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AMC (7-Amino-4-methylcoumarin) standard

Supplier: ENZO LIFE SCIENCES

Fluorogenic chromophore. Ex(max): 380nm, Em(max): 460nm. Reference compound.

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Nuclear-ID™ Blue/Red cell viability reagent (GFP-Certified™)

Supplier: ENZO LIFE SCIENCES

Cell viability dye

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femtoCHROMO™- HRP Substrate for chromogenic detection

femtoCHROMO™- HRP Substrate for chromogenic detection

Supplier: G-Biosciences

A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.

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Mix-n-Stain™ Cyanine dye antibody labelling kits

Mix-n-Stain™ Cyanine dye antibody labelling kits

Supplier: Biotium

Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.

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HRP - conjugate stabiliser, SeramunStab® STC

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.

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Nick Translation DNA Labelling System 2.0

Nick Translation DNA Labelling System 2.0

Supplier: ENZO LIFE SCIENCES

The Nick Translation DNA labelling system 2.0 provides a simple and efficient method for generating labelled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1 kb for fluorescent in situ hybridisation (FISH) applications.

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CF® Dye dCTP conjugates

Supplier: Biotium

CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

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Biotin reagents, OneQuant™ HOOK™

Supplier: G-Biosciences

The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.

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Amine reactive biotin reagents, HOOK™

Supplier: G-Biosciences

HOOK™ NHS-Biotin, 50 mg

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