Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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HEPES Buffer Solution (1 M)

Supplier: Stemcell Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Serva

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, PBS, powder pack for 5 litres of 10X

Supplier: Santa Cruz Biotechnology

Washing buffer, PBS, Powder pack for 5 litres of 10X

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Buffer, PBS solution 20X concentrate (phosphate buffered saline)

Supplier: Cell Signaling

Washing buffer, PBS buffer solution 20X concentrate (phosphate buffered saline)

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Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: Serva

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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Buffer, TRIS solution pH 8,8 (1 mol/L)

Supplier: Serva

Cloning Buffer, TRIS buffer solution pH 8,8 (1 mol/l)

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RoboSep™-C PBS/EDTA Buffer

Supplier: Stemcell Technologies

PBS buffer containing EDTA.

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer)

Supplier: Serva

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)

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Ammonium Chloride Solution

Supplier: Stemcell Technologies

Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: Ambion

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Intracellular Permeabilisation Buffer (10X)

Supplier: Stemcell Technologies

Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.

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Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++

Supplier: Stemcell Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: TREVIGEN

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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EasySep™ Red Blood Cell Lysis Buffer

Supplier: Stemcell Technologies

EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.