Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

pH Buffer solutions, CX-1

Supplier: Thermo Fisher Scientific

Delivers robust reproducible gradients with the Thermo Scientific™ pH buffer platform that is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Ambion

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: MO BIO

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Serva

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Ambion

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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RoboSep™ Buffer 2

Supplier: Stemcell Technologies

RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Molecular Biology Grade

Supplier: Serva

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, sodium acetate buffer solution pH 7 (3 mol/L)

Supplier: Serva

Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)

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ALDEFLUOR™ Assay Buffer

Supplier: Stemcell Technologies

Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.

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Fixation Buffer

Supplier: Stemcell Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.

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HEPES Buffer Solution (1 M)

Supplier: Stemcell Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Serva

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, PBS, powder pack for 5 litres of 10X

Supplier: Santa Cruz Biotechnology

Washing buffer, PBS, Powder pack for 5 litres of 10X

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

Supplier: Serva

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++

Supplier: Stemcell Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.