You searched for: qPCR/RT-qPCR Enzymes and Kits

Supplier: Biotium

Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).

Supplier: AGILENT

Improves reliability and yield of conventional Taq-based PCR amplifications.

Supplier: Quantabio

Deze kits bieden een eenvoudige en zeer gevoelige oplossing voor realtime reverse transcriptase-PCR (qRT-PCR) van RNA-templates door gebruik te maken van detectiechemie met hybridisatieprobes, zoals TaqMan® 5'-hydrolyseprobes.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Quantabio

PerfeCTa® qPCR ToughMix® is een 2X geconcentreerde, gebruiksklare reactiecocktail voor PCR-amplificatie van DNA-templates en lost veel bekende PCR-remmers op die vaak aanwezig zijn in ruwe monsters geëxtraheerd uit milieumonsters, plantaardig weefsel of dierlijk weefsel. Het is een veelzijdig en robuuste realtime qPCR-reagens die zorgt voor maximale gevoeligheid en PCR-efficiëntie bij uiteenlopende fluorogene sondechemie, zoals TaqMan® hydrolyseprobes. PerfeCTa® qPCR ToughMix® bevat alle benodigde reactiebestanddelen, behalve primers, probe(s), en DNA-templates. De lichtblauwe kleur van de AccuVue™ tracerkleurstof vereenvoudigt reactievorming in witte of heldere platen en draagt bij aan minimale pipetteer- of mengfouten. Het heeft geen invloed op de qPCR-prestaties of de stabiliteit van het product.

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® is een gebruiksklare, zeer gevoelige formule voor kwantitatieve omgekeerde transcriptie-PCR (RT-qPCR) van RNA-templates.

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: Quantabio

Deze SuperMix is een 2x geconcentreerde, gebruiksklare reactiecocktail voor realtime qPCR die alle componenten bevat behalve primers, probes en DNA templates. Het systeem overstijgt de multiplexbeperkingen van conventionele PCR-mengsels, waardoor zuivere amplificatie van maximale vijf targetsequenties in één buisje mogelijk is. Onderdrukking van ampliconen met een beperkt aantal kopieën door referentietargets met veel kopieën in de amplificatie is een veel voorkomend probleem bij multiplex-PCR. Hierdoor kan de schijnbare representatie en kwantificering van doelsequenties met een beperkt aantal kopieën worden vertekend of gemaskeerd. PerfeCTa® MultiPlex qPCR SuperMix geeft een dynamisch bereik en gevoeligheid aan multiplex-qPCR die vergelijkbaar zijn met die voor probe-analyses van enkelvoudig qPCR, zonder dat er beperkende of variabele primerconcentraties nodig zijn.

Supplier: AGILENT

Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: VWR Chemicals

VWR® Probe One-Step RT-qPCR Master Mix with UDG is designed for the detection and quantitation of RNA targets and is an ideal choice for multiplex applications. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: Quantabio

PerfeCTa® MultiPlex qPCR ToughMix® is een 5x geconcentreerde, gebruiksklare reactiemengsel voor real-time kwantitatieve PCR (qPCR) die veel bekende inhibitoren van PCR oplost die vaak aanwezig zijn in ruwe monsters die uit milieumonsters, plantenweefsels of dierlijke weefsels zijn geëxtraheerd. Het product bevat alle componenten, behalve primers, probes en templates. De 5x geconcentreerde ToughMix® maakt ook de toevoeging van grotere hoeveelheden sjabloon en verbeterde detectiegevoeligheid bij monsters met lage concentraties mogelijk. PerfeCTa® MultiPlex qPCR ToughMix® is geoptimaliseerd voor maximale PCR-efficiëntie, gevoeligheid en specificiteit in gereduceerde reactievolumes met snelle cycli of conventionele PCR-cyclusprotocollen.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Quantabio

qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

Supplier: AGILENT

High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: AAT BIOQUEST

TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

Supplier: AAT BIOQUEST

TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

Supplier: AAT BIOQUEST

A real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is a popular laboratory technique of molecular biology based on the polymerase chain reaction (PCR).

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.

Supplier: Thermo Fisher Scientific

Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.

Supplier: Thermo Fisher Scientific

The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: AAT BIOQUEST

ROX dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.

Supplier: FINNZYMES REAGENTS

DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.