You searched for: End-point PCR Enzymes and Kits

Supplier: Quantabio

Superior sensitivity and multiplexing for DNA amplification of long targets.

Supplier: ENZO LIFE SCIENCES

Optimised standard PCR mix for enhanced speed, yield, and specificity. Used for standard PCR applications.

Supplier: Quantabio

PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.

Supplier: Genscript

Taq DNA polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene.

Supplier: Quantabio

AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: AGILENT

This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

Supplier: AGILENT

Detect as low as 300 fg of Porcine DNA in food and pharmaceutical samples, including gel caps.

Supplier: ENZO LIFE SCIENCES

Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed, yield, and specificity.

Supplier: AGILENT

Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

Supplier: Quantabio

Superior speed and inhibitor tolerance.

Supplier: Apollo Scientific

Recombinant Taq plus DNA polymerase produced in E.coli 5 U/µl.

Supplier: AGILENT

High-quality polymerases for difficult to amplify DNA targets.

Supplier: AGILENT

This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

Supplier: Quantabio

The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

Supplier: AGILENT

Detects low copy number target and withstands PCR inhibitors without extensive purification.

Supplier: AGILENT

Paq5000 is capable of quickly and robustly amplifying up to 6 kB genomic DNA targets.

Supplier: Quantabio

AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

Supplier: AGILENT

PfuUltra High-Fidelity DNA Polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuUltra DNA Polymerase for robust, ultra-high-fidelity PCR.

Supplier: Biotium

Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq Gold by having a faster activation time and better shelf-life.

Supplier: Quantabio

AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: AGILENT

Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 s/kb, even with genomic DNA targets up to 23 kb.

Supplier: ENZO LIFE SCIENCES

Optimised standard PCR for enhanced speed, yield, and specificity.

Supplier: ENZO LIFE SCIENCES

Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.

Supplier: FINNZYMES REAGENTS

Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Supplier: FINNZYMES REAGENTS

Phusion® Green High-Fidelity DNA Polymerase is a combination of Phusion® DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Supplier: FINNZYMES REAGENTS

Phusion® Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion® Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

Supplier: FINNZYMES REAGENTS

Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.